百合‘Siberia’בManissa’种间杂交后代的染色体核型分析

以东方百合杂种系(Oriental hybrids group)品种‘Siberia’为母本,OT杂种系(interspecific hybrids betweenOriental and Longiflorum/OT group)品种‘Manissa’为父本杂交获得种间杂交F1代,对亲本及F1代株系的染色体数目和形态特征进行了分析。结果显示,母本东方百合‘Siberia’为二倍体即24条染色体,而父本‘Manissa’是高度杂合后代,为三倍体即36条染色体。杂交F1代的8个株系中有6个株系为二倍体即24条染色体,有2个株系为非整倍体,染色体条数分别为25和26条。母本‘Siberia’的核型为4m(1SAT)+10st(1SAT)+10t,父本‘Manis-sa’的核型为3m+18st(1SAT)+15t(1SAT),均属3B型。杂种F1代核型出现了多种类型,其中株系a、b、h为3B型,株系c、d、e、f为3A型,株系g为4B型。与亲本染色体形态相比,F1代株系出现了随体及端部着丝点染色体较多等染色体形态结构特征,而亲本没有这些特征。从染色体的形态、随体来看,子代为真杂种,在遗传上均偏向于母本。

Karyotype Analysis of the Interspecific Hybrids between Oriental Hybrids Group 'Siberia' and OT Group 'Manissa'

In this study,the karyotypes of Oriental hybrids group ’Siberia’,Oriental hybrids group ’Manissa’and their interspecific hybrids were analyzed to investigate the genetic characteristics.The results showed that female parent(’Siberia’) was diploid and male parent(’Manissa’) was triploid.6 hybrid lines F1 were diploid,2 hybrid lines F1 were aneuploid and their number of chromosomes was 25 and 26,respectively.The karyotype formula of ’Siberia’ was 4m(1SAT)+10st(1SAT)+10t belonging to ’3B’and ’Manissa’ was 3m+18st(1SAT)+15t(1SAT) belonging to ’3B’ according to Stebbins classification of karyotypic asymmetry.F1 showed different karyotypes,among lines a,b and h belonged to ’3B’,c,d,e and f belonged to ’3A’,and g belonged to ’4B’.F1 had satellite and more telocentric chromosomes,so they were true progeny,with a genetic tendency to their female parent.