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春兰内生真菌的分离及其抑菌活性的初步研究
引用本文:闵长莉,汪学军,刘文博.春兰内生真菌的分离及其抑菌活性的初步研究[J].西北植物学报,2012,32(3):596-599.
作者姓名:闵长莉  汪学军  刘文博
作者单位:1. 皖西学院生物与制药工程学院,安徽六安237012;植物细胞工程安徽省工程技术研究中心,安徽六安237012
2. 皖西学院生物与制药工程学院,安徽六安,237012
基金项目:六安市定向委托皖西学院市级研究项目,安徽省高等学校省级自然科学研究项目,国家自然科学基金项目
摘    要:采用平板分离法从皖西大别山野生兰花的根、茎、叶中分离内生真菌32株,用琼脂块法对获取的菌株进行抑菌活性筛选,其中有4种兰花内生真菌对供试菌株有抑菌作用,占总分离菌株的12.50%,对抑菌活性较强的一株内生真菌进行形态学鉴定,结果表明它属于无孢类群。

关 键 词:兰花  内生真菌  分离  抑菌活性

Preliminary Study on Isolation of Endophytic Fungi from Cymbidium goeringii and Its Antimicrobial Activity
MIN Chang-li , WANG Xue-jun , LIU Wen-bo.Preliminary Study on Isolation of Endophytic Fungi from Cymbidium goeringii and Its Antimicrobial Activity[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(3):596-599.
Authors:MIN Chang-li  WANG Xue-jun  LIU Wen-bo
Institution:1 (1 College of Biotechnology and Pharmaceutical Engineering,West Anhui University,Lu’an,Anhui 237012,China;2 Anhui Province Engineering Technology Research Center of Plant Cell Engineering,Lu’an,Anhui 237012,China)
Abstract:Thirty-two endophytic fungal strains were isolated from the roots,stems and leaves of wild Cymbidium goeringii by flat-panel separation.Agar block method was used to obtain the strain for antimicrobial activity screening.The results showed that four endophytic fungi(12.50% of the total strains isolated) had inhibitory activities to the test strains.We found a strong inhibitory activity of endophytic fungus.Morphological observation indicates that the strain belongs to Mycelia sterlia.
Keywords:Cymbidium goeringii  endophytic fungi  isolation  antimicrobial activity
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