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诱导子对百里香再生植株中苯丙氨酸解氨酶活性的影响
引用本文:杨 宁,李翠霞,李志忠,张 继.诱导子对百里香再生植株中苯丙氨酸解氨酶活性的影响[J].西北植物学报,2012,32(2):330-335.
作者姓名:杨 宁  李翠霞  李志忠  张 继
作者单位:1. 西北师范大学生命科学院,兰州,730030
2. 西北师范大学生命科学院,兰州730030;兰州理工大学生命科学与工程学院,兰州730050
3. 兰州理工大学生命科学与工程学院,兰州,730050
基金项目:国家自然科学基金,甘肃省科技厅自然科学基金,甘肃省教育厅基金,西北师范大学科技创新骨干项目
摘    要:以1/2MS为基本培养基,分别用蔗糖、NO3-/NH4+、苯丙氨酸、谷氨酸和酪氨酸诱导培养百里香组培苗30d,以及茉莉酸甲酯(MeJA)、水杨酸(SA)和壳聚糖诱导培养百里香20、30和40d组培苗植株0~120h,在不同的时间点测定其苯丙氨酸解氨酶(PAL)活性,以探讨诱导子对增殖百里香中PAL活性的影响。结果显示:以1/2MS为基本培养基,碳氮源调控30d后,百里香组培株的PAL活性以蔗糖浓度为2%、NO3-/NH4+比例为2∶1时最高;前体调控添加0.6mmol/L苯丙氨酸、0.8mmol/L谷氨酸和0.4mmol/L酪氨酸处理的百里香组培苗的PAL活性最高,分别为相应对照的126.3%、150.6%和153.9%。培养20d和30d植株的PAL活性均以1.0mmol/L MeJA、100mg/L SA和200mg/L壳聚糖诱导处理的最高,分别为相应对照的610.3%、788.1%、592.5%以及402.4%、541.2%、400.1%;而40d植株经0.5mmol/L MeJA、100mg/L SA和200mg/L壳聚糖诱导的PAL活性最高,分别为对照的390.2%、377.1%和413.4%。可见,在不同时期添加不同的诱导子对百里香再生植株中PAL活性有显著影响,且不同材料和诱导子存在各自适宜的诱导浓度和最佳诱导时间。

关 键 词:百里香  苯丙氨酸解氨酶  诱导子  酶活性

Effect of Elicitors on the Activity of L-Phenylalanine Transaminase in Plantlets of Thymus
YANG Ning,LI Cui-xi,LI Zhi-zhong,ZHANG Ji.Effect of Elicitors on the Activity of L-Phenylalanine Transaminase in Plantlets of Thymus[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(2):330-335.
Authors:YANG Ning  LI Cui-xi  LI Zhi-zhong  ZHANG Ji
Institution:1 (1 College of Life Science Northwest Normal University,Lanzhou 730030 China;2 College of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050 China)
Abstract:The plantlets of thyme were cultured on the 1/2 MS containing different sucrose,the NO3-/NH4+,phenylalanine,glutamic acid and tyrosine,and were tested for the PAL activity.The PAL activities were also tested after the plantlets were induced for 0~120 h by MeJA,SA and chitosan at different culture times(20 d,30 d,40 d).The results showed that the highest PAL activity was obtained when the plantlets of thyme were cultured on the 1/2 MS medium with 2% sugar concentration and 2∶1 NO3-/NH4+ ratio for 30 d.The PAL activities were increased to 126.3%,150.6% and 153.9% compared with CK by adding 0.6 mmol/L phenylalanine,0.8 mmol/L glutamic acid,and 0.4 mmol/L of tyrosine as precursor regulation,respectively.The PAL activities of plantlets cultured 20 d were increased 610.3%,788.1% and 592.5% than that of CK by adding 1.0 mmol/L MeJA,100 mg/L SA,and 200 mg/L chitosan as elicitors.The PAL activities of plantlets cultured 30 d were increased to 402.4%,541.2% and 400.1% compared with CK.And the highest PAL activities of plantlets cultured 40d were increased to 390.2%,377.1% and 413.4% than that of CK.Therefore,the PAL activity was affected by adding different inducer at different culture time.
Keywords:Thymus  L-phenylalanine transaminase  elicitor  enzyme activity
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