麻栎成熟合子胚外植体体胚发生和植株再生

为探索麻栎快速繁殖技术新途径,以成熟合子胚为外植体诱导体胚发生,进一步培养形成幼苗。结果表明:体胚诱导以MS+1.0mg.L-1 6-BA+1.0mg.L-1 IBA+1.0g.L-1谷氨酰胺+0.5g.L-1脯氨酸为最优,培养30d诱导率达70.0%;体胚成熟以1/2MS+2.0mg.L-1 6-BA+0.5mg.L-1 IBA+2.0mg.L-1 ABA+4.0g.L-1谷氨酰胺+2.0g.L-1脯氨酸为佳,培养60d,体胚完全成熟;体胚萌发最适培养基为1/2MS+0.2mg.L-1 6-BA+10g.L-1山梨醇,且冷处理有利于体胚的萌发,萌发率高达100%,萌发培养80d,形成再生植株。

Somatic Embryogenesis and Plant Regeneration from Mature Embryo Explants in Quercus acutissima Carr.

In order to explore a new approach to rapid propagation of Quercus acutissima Carr.,the somatic embryos were induced from the mature embryos.And then the somatic embryos were cultured into seedlings.The results revealed that the best medium to induce the somatic embryos was MS+1.0 mg·L-1 6-BA+1.0 mg·L-1 IBA+1.0 g·L-1 L-glutamine+0.5 g·L-1 proline on which the induction rate could reach to 70.0% in 30 d.The optimal mature medium was 1/2MS+2.0 mg·L-1 6-BA+0.5 mg·L-1 IBA+2.0 mg·L-1 ABA+4.0 g·L-1 L-glutamine+2.0 g·L-1 proline on which most of somatic embryos were fully mature in 60 d.The best germination medium was 1/2MS+0.2 mg·L-1 6-BA+10 g·L-1 sorbitol,and cold treatment was conducive for germination.The germination rate could reach to 100.0%.The regenerated plants formed in 80 d.