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| 植物抗病虫SSH文库的EST分析 | |
| 引用本文： | 王日升,张 曼,李 猷,黄凤婵,刘文君,郭晓燕.植物抗病虫SSH文库的EST分析[J].西北植物学报,2012,32(2):425-430. |
| 作者姓名： | 王日升 张 曼 李 猷 黄凤婵 刘文君 郭晓燕 |
| 作者单位： | 1. 广西农业科学院蔬菜研究所,南宁530007;广西作物遗传改良生物技术重点开放实验室,南宁530007 2. 广西农业科学院蔬菜研究所,南宁,530007 3. 广西作物遗传改良生物技术重点开放实验室,南宁530007;广西大学农学院,南宁530004 4. 广西农业科学院,南宁,530007 |
| 基金项目： | 广西自然科学基金重点项目,广西区青年科学基金,广西农业科学院基本科研业务专项,广西作物遗传改良生物技术重点开放实验室开放课题,广西农业科学院博士启动基金 |
| 摘 要： | 抑制差减杂交技术广泛应用于植物抗病虫(真菌、细菌和线虫)机理研究。本文在归纳出植物抗病虫SSH文库中ESTs的总体情况之基础上,重点分析了表达频率高的抗病、防御和信号转导基因,并展望了SSH的发展前景,有利于认识植物抗病虫分子机理的普遍规律和推广应用该技术。 |
| 关 键 词： | 抑制差减杂交 植物 抗病虫 表达序列标签 |
ESTs Analysis of Suppression Subtractive Hybridization Library from Plant Resistance to Disease and Insect Pest |
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| WANG Ri-sheng,ZHANG Man,LI You,HUANG Feng-chan,LIU Wen-jun,GUO Xiao-yan.ESTs Analysis of Suppression Subtractive Hybridization Library from Plant Resistance to Disease and Insect Pest[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(2):425-430. | |
| Authors: | WANG Ri-sheng ZHANG Man LI You HUANG Feng-chan LIU Wen-jun GUO Xiao-yan |
| Institution: | 1 Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;2 Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,Guangxi,China;3 Agricultural College,Guangxi University,Nanning 530004,Guangxi,China;4 Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China) |
| Abstract: | Suppression subtractive hybridization technology has been widely applied in the mechanism research of plant defense disease and insect pest,especially for fungi,bacteria and nematodes.The overall condition of ESTs from suppression subtractive hybridization libraries for plant resistance to disease and insect pest were generalized,and some highly expressed genes such as resistance genes,defense genes and signal transaction genes were analyzed emphatically.Finally,the future of SSH technology were reviewed.These helps to understand the universal rule of the mechanism of plant defense disease and insect pest,and enhance the application of suppression subtractive hybridization technology. |
| Keywords: | suppression subtractive hybridization plant ESTs of defense disease and pest expressed sequence tags |
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