小麦花粉管通道及子房注射法转化Anti-TrxS基因

为了获得抗穗发芽转基因小麦材料，以13个小麦品种(品系)为受体材料，用花粉管通道及子房注射法进行了硫氧还蛋白反义基因(Anti-TrxS)的遗传转化。花粉管通道法共转化小花2036朵，收获T0代种子1616粒，结实率为79．4％，将T0代种子大田点播，获T1代苗1424株，出苗率为88．1％。对T1代株系叶片基因组DNA进行PCR检测，31个株系中有16个株系呈阳性反应。子房注射法共转化小花1206朵，收获种子89粒，结实率为7．4％，T1代共出苗41株，出苗率为46．1％，对郑新991T1代株系进行PCR检测，电泳结果呈阳性反应。

Transforming Anti-TrxS gene into wheat by means of pollen tube pathway and ovary injection

In order to achieve a pre-harvest sprouting resistance wheat (Triticum aestivum L.), Anti-TrxS gene was transformed into 13 wheat cultivar receptors by means of pollen tube pathway and ovary injection. From 2 036 flowers transformed by pollen tube pathway, 1 616 seeds were achieved with a seed-set percentage of 79.4% and 1 424 T_1 plants were obtained with a seedling percentage of 88.1%. Leaf genome DNA from T_1 plant of different genotypes and treatments were analyzed by PCR and 16 positive lines were obtained among 31 tested lines. 89 seeds (7.4% of the flowers) and 41 T_1 plants (46.1% of seeds) were achieved from 1 206 flowers transformed by ovary injection. T_1 transgenic plant of Zhengxin 991 was identified by PCR analysis.