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兰科菌根真菌对铁皮石斛光合性能及pepc基因表达的影响
引用本文:魏 明,余茂元,柴瑞娟.兰科菌根真菌对铁皮石斛光合性能及pepc基因表达的影响[J].西北植物学报,2018,38(12):2276-2283.
作者姓名:魏 明  余茂元  柴瑞娟
作者单位:(安徽工程大学 生物与化学工程学院, 安徽芜湖 241000)
基金项目:安徽省自然科学基金(1408085MC65);
摘    要:为了阐明兰科菌根真菌对铁皮石斛光合作用的影响及机制,采用盆栽方式研究了兰科菌根真菌对铁皮石斛幼苗生长的影响,并分析了叶片中叶绿素含量、光合参数、叶绿素荧光参数以及pepc基因表达的变化。结果表明:(1)兰科菌根真菌促进了铁皮石斛幼苗生长,接种兰科菌根真菌的铁皮石斛的株高、根重、茎叶重和总生物量分别是未接种对照组的1.21、1.54、1.71和1.68倍;而且可显著提高叶片中叶绿素含量、叶片净光合速率(Pn)、蒸腾速率(Gs)和气孔导度(Tr)。(2)接种兰科菌根真菌的铁皮石斛叶片潜在光化学效率(Fv/F0)、最大光化学效率(Fv/Fm)、光化学猝灭系数(qP)、非光化学猝灭系数(qN)、实际光化学反应量子效率(Yield)和表观光合电子传递速率(ETR)均高于未接种对照组。(3)菌根真菌能促进pepc基因的表达,增强PEPC活性,提高铁皮石斛叶片的光合碳同化能力。研究表明,菌根的形成可以提高铁皮石斛叶片光合性能和pepc基因的表达水平,促进铁皮石斛幼苗的生长。

关 键 词:铁皮石斛  兰科菌根真菌  光合作用  pepc基因表达

Effect of Orchid Mycorrhizal Fungi on Photosynthetic Characteristics and pepc Gene Expression of Dendrobium officinale
WEI Ming,YU Maoyuan,CHAI Ruijuan.Effect of Orchid Mycorrhizal Fungi on Photosynthetic Characteristics and pepc Gene Expression of Dendrobium officinale[J].Acta Botanica Boreali-Occidentalia Sinica,2018,38(12):2276-2283.
Authors:WEI Ming  YU Maoyuan  CHAI Ruijuan
Abstract:In order to find out the impact and mechanism of orchid mycorrhizal (OM) fungi on photosynthesis of Dendrobium officinale, we investigated the effect of OM fungi on the growth of D. officinale seedlings using a pot experiment. The chlorophyll content, photosynthetic characteristics, chlorophyll fluorescence parameters, and the expression of pepc gene were analyzed. The results were as follows: (1) OM fungi could improve the growth of D. officinale seedlings. The height of plants, mass of roots, mass of shoots and total biomass of D. officinale seedlings were of 1.21, 1.54, 1.71 and 1.68 fold, respectively, compared to control. Chlorophyll content, net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of D. officinale seedlings increased too. (2) The potential photochemical efficiency (Fv/F0), photochemical maximum efficiency (Fv/Fm), the photochemical quenching coefficient (qP), non photochemical quenching coefficient (qN), quantum yield of electron transport (Yield) and apparent photosynthetic electron transport rate (ETR) in leaves of D. officinale seedlings inoculated with OM fungi were higher than that of control. (3) OM fungi could enhance the expression level of pepc gene, PEPC activity, and promote photosynthetic carbon assimilation of D. officinale. Therefore, the development of D. officinale mycorrhiza could improve photosynthesis, the expression level of pepc gene and the growth of D. officinale seedlings.
Keywords:Dendrobium officinale  orchid mycorrhizal fungi  photosynthesis  pepc gene expression
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