大豆GmABI4基因克隆及表达分析

该研究基于大豆基因组数据库,根据拟南芥ABI4蛋白的氨基酸序列,经比对分析,获得了大豆中的2个GmABI4基因,分别命名为GmABI4-1(GenBank登录号为XM_014766551.1)和GmABI4-2(GenBank登录号为NM_001249003)。TMHMM软件和系统进化转录分析表明,这2个基因编码的蛋白均不具有信号肽,二级结构主要以无规则卷曲和延伸链为主;进化树分析表明,大豆GmABI4和野生大豆亲缘关系较近。荧光定量PCR分析表明,GmABI4-1与GmABI4-2基因在大豆种子与豆荚中的表达量均高于根、茎、叶、花等其他组织,推测可能与调控大豆种子生命活动相关。

Cloning and Expression Analysis of GmABI4 Gene in Soybean

This study was based on the soybean genome database. According to the amino acid sequence of Arabidopsis thaliana ABI4 protein, we obtained two GmABI4 genes in soybean genome and named GmABI4 1 (GenBank accession number XM_014766551.1) and GmABI4 2 (GenBank accession number NM_001249003), respectively. The TMHMM software and phylogenetic analysis showed that the proteins encoded by these two genes had no signal peptides, and their secondary structures mainly consisted of extended strand and random coil. Phylogenetic analysis revealed that soybean GmABI4 was closely related to that of wild soybean. The results of real time quantitative PCR analysis showed that the expression levels of GmABI4 1 and GmABI4 2 in seeds and soybean meal were higher than those in roots, stems, leaves, flowers and other tissues, which suggest that GmABI4 is probably involved in seed biology.