离子束介导大豆DNA转化小麦后代高蛋白株的RAPD标记分析

利用离子束介导法将大豆DNA导入小麦,经过连续4代田间筛选和蛋白含量测定,获得高蛋白变异株系.采用RAPD分析技术,用34条随机引物对供体大豆、受体小麦和3个高蛋白小麦变异株的基因组DNA进行扩增.有29个引物扩增出清晰稳定的条带,其中18个引物扩增出的条带有不同程度的差异.高蛋白小麦突变株与受体小麦(对照)相比出现了条带的增加、缺失、扩增带深浅等变化,也出现了与受体小麦不同而与供体大豆相同的扩增带.实验结果表明,外源大豆DNA导入受体小麦可以引起后代基因组DNA序列变化,扩大小麦遗传基础.

RAPD Marker Analysis of High-protein Offspring Plants of Wheat Transformed with Soybean DNA through Particle Beam Mediation

Wheat was transformed with soybean DNA through particle beam mediation and its high-protein offspring plants were obtained through field selections and protein determinations in the four consecutive generations.34 random primers was employed to amplify genomic DNA of the donor soybean,the receptor wheat and three high-protein wheat plants,and clear-cut bands were amplified with 29 of them and the bands amplified with 18 of the primers differed in various degrees.Compared with the receptor wheat the high-protein plants appeared to add new bands,lose bands and vary in the intensity of amplified bands as well as presented the amplified bands,which were different from those in the receptor wheat and the same as those in the donor soybean.These results showed that the transformation of exogenous DNA of soybean into wheat could result in the offspring of the receptor wheat varying in their DNA sequences thus enlarging the genetic basis of wheat.