| 二甲戊灵对离体大蒜染色体的加倍研究 |
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| 引用本文： | 周香君,程智慧,孟焕文.二甲戊灵对离体大蒜染色体的加倍研究[J].西北植物学报,2009,29(12). |
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| 作者姓名： | 周香君 程智慧 孟焕文 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西杨陵,712100 |
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| 基金项目： | 国家公益性行业(农业)科研专项，西北农林科技大学唐仲英育种基金 |
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| 摘 要： | 在MS分化培养基中分别添加不同浓度的秋水仙素和二甲戊灵,以 "改良蒜" 的蒜瓣基部为外植体诱导愈伤组织染色体变异并再生植株,观察根尖细胞染色体数和叶片下表皮保卫细胞特征检测诱变效果.结果表明:大蒜染色体数变异受诱变剂浓度和处理持续时间的影响,诱变剂高浓度和短时间处理均有利于大蒜愈伤组织的分化;添加0.1%秋水仙素处理5 d,愈伤组织存活率为80.7%,分化率为78.1%,四倍体的诱导率为4%;添加 100 μmol/L二甲戊灵处理5 d,愈伤组织存活率为100%,四倍体的诱导率为6%.对根尖细胞染色体和叶片下表皮气孔数目和大小观察显示,诱变株具有四倍体的基本特征.说明二甲戊灵能在离体条件下有效诱导大蒜产生四倍体,可替代秋水仙素对大蒜染色体的加倍作用.
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| 关 键 词： | 大蒜 秋水仙素 二甲戊灵 染色体加倍 |
Effects of Pendimethalin on Garlic Chromosome Doubling in Vitro |
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| ZHOU Xiang-jun,CHENG Zhi-hui,MENG Huan-wen.Effects of Pendimethalin on Garlic Chromosome Doubling in Vitro[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(12). |
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| Authors: | ZHOU Xiang-jun CHENG Zhi-hui MENG Huan-wen |
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| Institution: | ZHOU Xiang-jun,CHENG Zhi-hui,MENG Huan-wen(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | Callus induced from garlic clove stem cv.Gailiang was used to induce chromosome variation in vitro with colchicine or pendimethalin at three different concentrations added in the MS culture medium.The effects of induction were identified by observing the chromosome number in root tip cells and the guard cell characteristics on lower epidermis of the induced plantlet leaves.The results show that the chromosome variation is influenced by the concentration of inducing agents and the duration of induction.Highe... |
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| Keywords: | garlic (Allium sativum L ) colchicine pendimethalin chromosome doubling |
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