甜瓜乙烯应答因子基因在果实发育成熟过程中的表达特性

根据已报道的甜瓜CMe-ERF1和CMe-ERF2基因cDNA序列设计合成特异性引物,应用RT-PCR技术从甜瓜品种‘河套蜜瓜’成熟果实中克隆得到CMe-ERF1和CMe-ERF2基因cDNA全长编码区序列,分别为498bp和822bp.序列比对分析表明,得到的cDNA序列与已报道的Andes甜瓜相应基因的cDNA序列完全一致.果实不同发育时期实时定量PCR检测结果表明,CMe-ERF1、CMe-ERF2基因表达与甜瓜果实成熟及乙烯生成量显著相关,表明该基因可能对果实成熟起重要作用.

Expression Characterization of Ethylene Response Factor Gene CMe-ERF during Development and Ripening of Melon(Cucumis melo L.)Fruit

Two pairs of gene specific primers were designed according to the nucleotide sequence of CMe-ERF1 and CMe-ERF2 genes from melon in GenBank.The full-length coding regions of the CMe-ERF1 and CMe-ERF2 cDNA,which were 498 bp and 822 bp respectively,were cloned by RT-PCR from ripening fruit of melon cv.Hetao.Sequence analysis indicated that the cDNA sequences were consistent with that of reported melon CMe-ERFs cDNA.Expression levels of CMe-ERF1 and CMe-ERF2 mRNA were investigated at different development stages of melon fruit by qPCR,and showed that the expression levels of these genes were highly relative to fruit ripening and ethylene production.The results indicate the genes might play important role in fruit ripening.