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| 鸭梨PbHCT3基因的克隆及表达分析 | |
| 引用本文： | 葛文雅,惠 伟,闫洪波,程玉豆,关军锋.鸭梨PbHCT3基因的克隆及表达分析[J].西北植物学报,2012,32(5):871-875. |
| 作者姓名： | 葛文雅 惠 伟 闫洪波 程玉豆 关军锋 |
| 作者单位： | 1. 陕西师范大学生命科学学院,西安710062;河北省农林科学院遗传生理研究所,石家庄050051;河北省植物转基因研究中心,石家庄050051 2. 陕西师范大学生命科学学院,西安,710062 3. 河北省农林科学院遗传生理研究所,石家庄050051;河北经贸大学生物科学与工程学院,石家庄050061;河北省植物转基因研究中心,石家庄050051 4. 河北省农林科学院遗传生理研究所,石家庄050051;河北省植物转基因研究中心,石家庄050051 |
| 基金项目： | 国家现代农业（梨）产业技术体系建设专项资助(CARS-29) |
| 摘 要： | 羟基肉桂酰CoA:莽草酸/奎宁酸羟基肉桂酰转移酶(HCT)是植物绿原酸合成的关键酶之一.该研究以鸭梨为材料,采用同源基因克隆的方法,克隆出一个鸭梨的HCT基因,命名为PbHCT3.PbHCT3基因cDNA全长序列为1 731bp,基因编码序列长度为1 317bp,编码438个氨基酸,含有酰基转移酶的2个保守序列HHAAD和DFGWG及保守结构域MVVNVTVRES.实时荧光定量PCR分析表明:PbHCT3基因在幼果果皮、果肉、果心、幼叶及花蕾期花瓣中的表达量较高.随着鸭梨果实生长,果实中PbHCT3基因表达量逐渐降低.研究表明,PbHCT3基因与鸭梨幼嫩组织的生长发育有密切关系. |
| 关 键 词： | 鸭梨 羟基肉桂酰CoA:莽草酸/奎宁酸羟基肉桂酰转移酶 酰基转移酶 基因克隆 绿原酸 |
Cloning and Expression Analysis of PbHCT3 Gene in Pyrus bretschneideri Rehd.cv.'Yali' |
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| GE Wen y,HUI Wei,YAN Hong bo,CHENG Yu dou,GUAN Jun feng.Cloning and Expression Analysis of PbHCT3 Gene in Pyrus bretschneideri Rehd.cv.'Yali'[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(5):871-875. | |
| Authors: | GE Wen y HUI Wei YAN Hong bo CHENG Yu dou GUAN Jun feng |
| Institution: | 2,4(1 College of Life Science,Shaanxi Normal University,Xi’an 710062,China;2 Institute of Genetics and Physiology,Hebei Academy of Agricultrue and Forestry Sciences.Shijiazhuang 050051,China;3 Bioscience and Bioengineering School,Hebei University of Economy and Business,Shijiazhuang 050061,China;4 Plant Genetic Engineering Center,Hebei Academy of Agricultrue and Forestry Sciences,Shijiazhuang 050051,China) |
| Abstract: | Hydroxycinnamoyl-coenzyme A:shikimate/quinate hydroxycinnamoyltransferase(HCT) is one of the key enzymes in plant chlorogenic acid synthesis.In this study,we isolated HCT gene by homologous cloning method from ’Yali’,and named as PbHCT3.The full length of PbHCT3 cDNA is 1 731 bp,and the 1 317 bp open reading frame encodes 438 amino acids which containing two conservative sequences HHXXD and DFGWG and conservative structure MVVNVTVRES domain of acyltransferase.Real-time PCR analysis indicated that PbHCT3 showed a higher expression in peel,pulp and core of young fruit,young leaves,and alabastrum,but the expression in fruit decreased with the fruit growth.These results suggested the PbHCT3 is closely related to growth and development of younger tissue in ’Yali’. |
| Keywords: | Yali HCT acyltransferase gene cloning chlorogenic acid |
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