节节麦-黑麦双二倍体种子贮藏蛋白的变异分析

利用SDS-PAGE和A-PAGE方法对获得的遗传稳定性逐年提高节节麦-黑麦双二倍体的麦谷蛋白和麦醇溶蛋白进行了分析.结果显示:在高分子量谷蛋白区域,双二倍体共检测到3条带,其中第1和第3条带与亲本节节麦的5t和10t亚基大小一致,中间的第2条带是两亲本都未出现的新麦谷蛋白条带,而黑麦的2r和6.5r亚基在双二倍体材料中未检测到;在低分子量谷蛋白区域,黑麦和节节麦分别有4条和2条带在双二倍体材料中未检测到.在醇溶蛋白的γ和β区,黑麦共有5条带在双二倍体材料中未检测到,在ω区,节节麦有1条带未检测到.研究表明,在双二倍体中两亲本控制贮藏蛋白的基因组之间发生了较大的改变,与亲本节节麦相比较,亲本黑麦控制的贮藏蛋白发生的改变更大;两亲本控制贮藏蛋白基因的遗传方式在双二倍体材料中表现为非加性效应.

Variation Analysis of Seed Storage Proteins among Advanced Aegilops tauschii × Secale cereale Amphipioids

The glutenin and gliadin in the Aegilops tauschii×Secale cereale amphiploid with increasing genetic stability were analyzed by SDS-PAGE and A-PAGE methods.The results showed that 3 bands of the amphiploid were detected in the HMW region,where in the first and the third bands have the same size with the 5t and 10t subunits of the parent A.tauschii.The second band was a new glutenin band which could not be found from its two parents.2r and 6.5r subunits of S.cereale could not be found in the amphiploid.In the LMW region,4 and 2 bands were not observed in their corresponding amphiploid for S.cereale and A.tauschii,respectively.In the γ and β regions of gliadin,there were altogether 5 bands of S.cereale not detected in the amphiploid and only 1 band was missed for A.tauschii in the ω region.The above results indicated that great changes have taken place between the two parental genomes controlling storage proten in the amphiploid.Compared with that of A.tauschii,greater changes have experienced in the storage proteins controlled by S.cereale.The inheritance mode of storage protein genes in the amphiploid appeared non-additive effects.