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| 小麦温敏雄性不育系YM3314育性转换相关基因分析 | |
| 引用本文： | 李红燕,周琳璘,何蓓如,胡银岗.小麦温敏雄性不育系YM3314育性转换相关基因分析[J].西北植物学报,2009,29(11). |
| 作者姓名： | 李红燕 周琳璘 何蓓如 胡银岗 |
| 作者单位： | 1. 西北农林科技大学,农学院,陕西杨陵,712100;济宁学院,生物系,山东曲阜,273100 2. 西北农林科技大学,农学院,陕西杨陵,712100 3. 西北农林科技大学,农学院,陕西杨陵,712100;陕西省农业分子生物学重点实验室,陕西杨陵,712100;国家小麦改良中心杨凌分中心,陕西杨陵,712100 |
| 基金项目： | 教育部重点科研项目，教育部春晖计划，国家科技支撑计划，陕西省科技攻关计划 |
| 摘 要： | 采用抑制消减杂交技术,构建了小麦温敏不育系YM3314在陕西杨陵秋播(不育环境)和春播(可育环境)幼穗单核期正反交cDNA文库,对获得的136条高质量EST序列采用BLASTx序列比对进行同源性分析,并利用AmiGo和QuickGo浏览器根据GO(gene ontology)数据库对基因表达产物进行功能分类,比较两种育性条件下幼穗cDNA文库的表达谱,以探讨YM3314的育性转换机理.结果显示:不育文库(B库)中初级代谢(13.51%)、转运(13.51%)、抗病与防御(8.11%)以及功能未分类(18.92%)的EST序列所占比例均高于可育文库(K库)中对应的EST序列比例,且分别是K库的2.30倍、1.53倍、2.76倍、1.29倍;K库中与信号传递(14.71%)相关的EST序列所占比例明显高于B库,是B库的2.72倍;两个库中与能量代谢、蛋白质合成、蛋白质修饰/加工/储藏相关的EST序列所占比例相差不大.分析表明,两个文库中与初级代谢、信号传递等有关的基因表达差异可能是导致其育性转换的重要基础. |
| 关 键 词： | 小麦 温敏雄性不育 抑制消减杂交 表达序列标签 |
Analysis on the Genes Related to Fertility Conversion in Wheat Thermo-sensitive Male-sterile Line YM3314 |
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| LI Hong-yan,ZHOU Lin-lin,HE Bei-ru,HU Yin-gang,.Analysis on the Genes Related to Fertility Conversion in Wheat Thermo-sensitive Male-sterile Line YM3314[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(11). | |
| Authors: | LI Hong-yan ZHOU Lin-lin HE Bei-ru HU Yin-gang |
| Institution: | LI Hong-yan1,2,ZHOU Lin-lin1,HE Bei-ru1,HU Yin-gang1,3,4* (1 College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China,2 Department of Biology,College of Jining,Qufu,Sh,ong 273100,3 Key Labaratery of Molecular Biology for Agriculture of Shaanxi Province,4 Yangling Branch of China National Wheat Improvement Centre,China) |
| Abstract: | Using suppression subtractive hybridization(SSH),cDNA libraries of the young spikes of uninucleate stage of YM3314 which were sowed in autumn(sterile conditions) and spring(fertile conditions) were constructed,and 136 high quality sequences were obtained.Sequence homology analyses were conducted by BLASTx in NCBI against nr-Protein database of GenBank.AmiGo and QuickGo browser by GO database were used to functional classification for gene products.Comparison analysis proceeded on the gene expression profile... |
| Keywords: | wheat(Triticum aestivum L ) thermo-sensitive male-sterility suppression subtraction hybridization(SSH) expressed sequence tag(EST) |
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