羊草光合作用相关基因的克隆与分析

在构建了羊草叶片cDNA文库的基础上,利用M13载体通用引物筛选其亚文库,挑选阳性克隆进行测序,将测序结果在NCBI基因库中进行比对,得到一个Rubisco大亚基基因全长序列和Rubisco小亚基基因部分序列,并对其核苷酸及其编码的氨基酸序列进行分析。结果显示,Rubisco大亚基基因长度为1 796 bp,与禾本科大麦、小麦、野雀麦、粗山羊草、旱麦草、异形花草、黑麦等的核苷酸序列同源性达98%以上;羊草的Rubisco小亚基基因部分序列含有一个开放阅读框,其长度为186 bp,编码61个氨基酸,与禾本科的小麦、大麦、燕麦、黑麦以及扁穗雀麦Rubisco小亚基基因氨基酸序列的同源性分别为93%、93%、91%、91%、92%。羊草Rubisco基因的克隆与分析有利于进一步研究其光合作用效率。

Cloning and Analysis of Leymus chinensis Photosynthesis Related Genes

Based on the leaf cDNA library of Leymus chinensis,we used the M13 universal primers to screen positive clones to sequence.The sequences were submitted to NCBI for estimating their homogeneity,and we got a Rubisco large subunit gene and a gene fragment of Rubisco small subunit.Rubisco large subunit gene was 1 796 long,which was more than 98% homologous to the sequences of Rubisco large subunit gene nucleotides in Hordeum capense,Triticum aestivum,Bromus arvensis,Aegilops tauschii,Eremopyrum triticeum,Heteranthelium piliferum et al.ORF of Rubisco small subunit gene was 186 bp long and encoded 61 amino acids.It was 93%,93%,91%,91% and 92% homologous to the sequences of amino acid in Hordeum capense,Triticum aestivum,Avena clauda,Secale cereale and Bromus cathartic,respectively.The cloning of Rubisco genes is helpful for the further research of photosynthetic efficiency in Leymus chinensis.