羊草与灰色赖草F1代细胞悬浮系的建立及植株再生

本研究以羊草(L eym us ch inensis)与灰色赖草(L eym us cinereus)杂种F1代幼穗为外植体诱导愈伤组织,在3.0 m g/L 2,4-D M S培养基上继代1次后,转入不同浓度激素(2,4-D、IAA、KT)配比和不同浓度蔗糖的M S液体培养基进行振荡培养,建立杂种F1代细胞悬浮系和植株再生体系.结果表明,细胞悬浮培养时,M S 1.0 m g/L2,4-D 0.1 m g/L KT 4%蔗糖的液体培养基最佳;悬浮细胞分化时,1.0 m g/L 2,4-D 0.1 m g/L KT 4%蔗糖 M S和1.0 m g/L 2,4-D 4%蔗糖 M S培养的悬浮细胞在1.0 m g/L NAA 0.5 m g/L KT M S分化培养基上的绿苗分化率分别达到83%和80%.细胞悬浮系及再生体系的建立为杂种F1代育性恢复的研究奠定了基础.

Construction and Plant Regeneration of F1 Cell Lines of Suspension Culture between Leymus chinensis and Leymus cinereus

The study induced F_1 young spikes between Leymus chinensis and L.cinereus to produce callus and then sub-cultured it on the medium of MS 3.0 mg/L 2,4-D.Next,it was transferred to MS medium with combined different combined hormone concentrations(2,4-D,IAA and KT) and sucrose concentrations and cultured in a shaking mode to develop the cell lines of suspension culture and the system of plant regeneration in the F_1.Results indicated that the liquid medium of MS 1.0 mg/L 2,4-D 0.1 mg/L KT 4% sucrose was the best in the suspension culture;in the differentiation,the suspension cells cultured on the medium of MS 1.0 mg/L 2,4-D 0.1 mg/L KT 4% sucrose and 1.0 mg/L 2,4-D 4% sucrose MS differentiated into seedlings on the differentiation medium of 1.0 mg/L NAA 0.5 mg/L KT MS at the rates of 83% and 80%,respectively.The development of the suspension cells and plant regeneration would lay a foundation for the fertility restoration in F_1.