| 制备高分辨植物SEM样品的微波浸软法 |
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| 引用本文： | 高德禄,张和民.制备高分辨植物SEM样品的微波浸软法[J].西北植物学报,1993,13(1):58-62. |
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| 作者姓名： | 高德禄 张和民 |
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| 作者单位： | 中国科学院植物研究所，中国科学院植物研究所 北京 100044，北京 100044 |
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| 摘 要： | 微波辐射浸软法是一种简单、快速制备高分辨植物扫描电镜样品的方法。经冰冻断裂处理后的样品,利用断续微波辐射浸软,既可阻止浸软溶液温度陡然升高,不使植物结构受到破坏,又可使浸软时间显著缩短,由原来的72小时变为30分钟。因为断续的微波辐射能保持较低的炉内温度,所以它不仅能很好地适用于扫描电镜样品制备过程中的浸软,而且也适用于样品固定、导电染色等。此法大大缩短了O-D-O法的制样周期。
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| 关 键 词： | 微波侵软法 植物样品 电子显微镜 |
THE MACERATION METHOD FOR PREPARATION OF PLANT SPECIMEN OF HIGH RESOLUTION FOR SCANNING ELECTRON MICROSCOPY USING MICROWAVE |
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| Gao Delu and Zhang Hemin.THE MACERATION METHOD FOR PREPARATION OF PLANT SPECIMEN OF HIGH RESOLUTION FOR SCANNING ELECTRON MICROSCOPY USING MICROWAVE[J].Acta Botanica Boreali-Occidentalia Sinica,1993,13(1):58-62. |
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| Authors: | Gao Delu and Zhang Hemin |
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| Abstract: | Microwave irradiation was applied to maceration of cells of onion root tip meristem after freeze-cracking for scanning electron microscopy. Intermittent microwave irradiation prevented suddenly rise in the solution temperature, from causing damage to tissue specimens during maceration. The maceration time was strikingly shortened from 72 h to 30 min. The intermittent microwave irradiation method, which keeps the temperature low, may be well useful for the sampling procedures including conductive staining. The preparation period of O-D-O method was strikingly shortened. |
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| Keywords: | microwave irradiation organelles osmium-dimethyl sulfoxide-osmium method scanning electron microscopy(SEM) |
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