长春花钙调素基因克隆及其假基因的识别

以长春花Catharanthus roseus(L.)G.Don]叶片cDNA和基因组DNA为模板,利用PCR技术扩增得到了长春花钙调素基因447 bp的全长编码cDNA序列和2个大小不同的DNA片段.序列分析表明,DNA长片段全长1 551 bp,由2个外显子和1个内含子构成,为长春花钙调素基因编码区DNA片段;DNA小片段全长447 bp,与447 bp的长春花钙调素基因cDNA核苷酸一致性高达87%,有56个碱基的差异,其中位于226 bp处的碱基A突变为T,即由AAG突变为终止密码子TAG使翻译提前终止.推测此447 bp的DNA小片段可能为长春花钙调素基因的假基因,命名为CCaMP1.

Molecular Cloning of A Calmodulin Gene and Identification of Its Pseudogene in Catharanthus roseus

A calmodulin gene 447 bp-length cDNA and two DNA fragments were isolated with cDNA and genome DNA extracted from Catharanthus roseus leaves as templates.Two DNA fragments were sequenced indicating the 1 551 bp-length DNA fragment is CaM gene containing one intron and two exons,and the 447 bp-length DNA fragment has 87% identity with CaM gene cDNA in C.roseus including 56 different nucleotides.The 447 bp-length DNA fragment,in which A was mutated to T at 226 bp site leading to stop codon TAG in advance,was suggested to be a pseudogene of CaM gene in C.roseus plant,named CCaMP1.