| 基于DNA序列的白头翁属植物自然杂交证据 |
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| 引用本文： | 杨威,邱爽,金武烈,吕惠子.基于DNA序列的白头翁属植物自然杂交证据[J].西北植物学报,2011,31(9). |
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| 作者姓名： | 杨威 邱爽 金武烈 吕惠子 |
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| 作者单位： | 1. 延边大学药学院,吉林延吉,133000
2. 韩国忠北大学生命科学学院,韩国全州,561756 |
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| 基金项目： | 吉林省中医药管理局中医药科技项目(08sys-101) |
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| 摘 要： | 对长白山区兴安白头翁(Pulsatilla dahuricaSprengel)、朝鲜白头翁P.cernua(Thunb.)Berchtold&Presl]以及其自然杂交种(P.dahurica×P.cernua)进行ITS和psbA-trnH序列分析,结果表明,自然杂交种与兴安白头翁遗传距离为0;ITS电泳显示,在6个信息位点的峰值同时具有朝鲜白头翁和兴安白头翁信息的双重峰,进一步证实前者是朝鲜白头翁和兴安白头翁的自然杂交种.
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| 关 键 词： | 白头翁 自然杂交 nrITS psbA-trnH |
Identification of Natural Hybrid among Pulsatilla Based on DNA Sequences |
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| YANG Wei,QIU Shuang,JIN Wu-lie,L Hui-zi.Identification of Natural Hybrid among Pulsatilla Based on DNA Sequences[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(9). |
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| Authors: | YANG Wei QIU Shuang JIN Wu-lie L Hui-zi |
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| Institution: | YANG Wei1,QIU Shuang1,JIN Wu-lie2,Lü Hui-zi1(1 College of Pharmacy,Yanbian University,Yanji,Jilin 133000,China,2 Division of Biological Sciences,Chonbuk National University,Jeonju 561756,Korea) |
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| Abstract: | A natural hybrid between Pulsatilla dahurica and P.cernua was genetically identified by sequence analyses of nuclear Internal Transcribed Spacer(ITS) region and chloroplast psbA-trnH gene.The natural hybrid displayed double peaks in the sequence chromatogram at 6 diagnostic positions.That indicating the species was derived from the two parental haploid genomes.Meanwhile,comparison of psbA-trnH intergenic spacer sequences of chloroplast DNA,which are well characterized by the maternal inheritance pattern,sho... |
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| Keywords: | Pulsatilla natural hybrid nrITS psbA-trnH |
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