暗培养对黄瓜子叶节再生频率及其相关酶活性和可溶性蛋白含量的影响

以黄瓜品种新泰密刺子叶节为材料,观测了暗培养1~5 d黄瓜子叶节植株再生频率及其诱导过程中POD、SOD活性和可溶性蛋白含量的变化,以探究暗培养条件下黄瓜子叶节POD、SOD活性、可溶性蛋白含量与再生频率间的关系.结果表明,暗培养不仅可以使黄瓜子叶节POD活性持续增长,还有助于其活性保持在较高水平,D4处理在培养的第6天POD值高达243 U?g-1FW,对应的再生频率为87.20%,而对照的最高峰值仅为187.7 U?g-1FW,再生频率只有36.60%;POD的变化体现了各处理生理状态的差异,且各处理暗培养结束时的POD活性水平和POD峰值分别与植株再生频率之间存在显著正相关性,相关系数分别达到了0.921和0.839;而SOD活性水平与再生频率间无显著相关性;可溶性蛋白含量虽然可以体现子叶节生理状态的变化,但无法反映各暗处理对再生频率的影响差别.可见,暗培养有助于提高诱导过程中黄瓜子叶节POD活性的增速,使其活性保持在较高水平,且以POD活性为指标可以反映暗培养处理的有效性.

Effect of Dark Culture on Enzyme and Soluble Protein Variance during Cucumber Cotyledon Node Regeneration

The relationship between regeneration frequency and POD activity as well as SOD activity and soluble protein content was studied by cucumber cultivar 'Xintaimici'.The results showed that the dark cultivation could not only contribute to POD activity grow,but also conduce to its activity remained at a high level.The change of POD activity reflected physiological differences between treatments.The correlation analysis showed that POD activity at the end of dark cultivation,POD activity peaks were significantl...