| 云南松胚性愈伤组织诱导及增殖 |
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| 引用本文： | 于大德,肖宁,王企珂,王晟,胡冬梅,张金凤.云南松胚性愈伤组织诱导及增殖[J].西北植物学报,2011,31(10):2119-2123. |
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| 作者姓名： | 于大德 肖宁 王企珂 王晟 胡冬梅 张金凤 |
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| 作者单位： | 北京林业大学林木育种国家工程实验室,林木、花卉遗传育种教育部重点实验室,国家林业局树木花卉育种与生物工程重点开放实验室,北京100083 |
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| 基金项目： | 行业专项(201004009); 国家大学生创新性实验计划(091002212); 中央高校基本科研业务费专项资金资助 |
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| 摘 要： | 以云南松成熟合子胚为外植体,在DCR培养基上诱导胚性愈伤组织,探索最佳消毒剂用量及激素浓度配比。用不同的培养方法增殖胚性愈伤组织,并对胚性愈伤组织形成过程中形态学与细胞学变化进行观察。其后提高培养基中肌醇浓度,增大渗透压,添加ABA,诱导早期原胚。结果表明,经5%次氯酸钠消毒20 min,使用添加8mg/L 2,4-D1、mg/L 6-BA、500 mg/L CH以及500 mg/L谷氨酰胺的DCR培养基接种,诱导率较高,可达70%以上;采用在愈伤组织周围添加少量培养过该愈伤组织的培养基的方式继代,愈伤组织增殖率可由20%显著提高至50%左右。在增殖培养基中添加4~6 mg/L ABA后,胚性细胞可逐渐发育形成胚性胚柄团。
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| 关 键 词： | 云南松 体细胞胚胎发生 诱导 增殖 条件培养 |
Induction and Proliferation of Embryo Callus from Pinus yunnanensis Franch. |
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| YU Da-de,XIAO Ning,WANG Qi-ke,WANG Sheng,HU Dong-mei,ZHANG Jin-feng.Induction and Proliferation of Embryo Callus from Pinus yunnanensis Franch.[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(10):2119-2123. |
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| Authors: | YU Da-de XIAO Ning WANG Qi-ke WANG Sheng HU Dong-mei ZHANG Jin-feng |
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| Institution: | YU Da-de,XIAO Ning,WANG Qi-ke,WANG Sheng,HU Dong-mei,ZHANG Jin-feng*(Beijing Forestry University,National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,Beijing 100083,China) |
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| Abstract: | This article discusses the optimum induction condition of embryonic callus from Yunnan pine(Pinus yunnanensis Franch.) mature embryos treated with various concentration of 2,4-D and 6-BA on DCR medium after sterilizing with sodium hypochlorite solution.The morphological and cytological observation of the development of somatic embryogenesis is conducted,and various culture methods are used in the step of proliferation.Early proembryos were induced by adding ABA with high osmotic pressure of the medium.The r... |
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| Keywords: | Yunnan pine somatic embryogenesis induction proliferation conditional culture |
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