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羽衣甘蓝花粉母细胞减数分裂及雄配子体发育观察
引用本文:魏跃,赵桂华,杨鹤同,朱耀,李玉进,张蜀宁.羽衣甘蓝花粉母细胞减数分裂及雄配子体发育观察[J].西北植物学报,2011,31(9):1753-1757.
作者姓名:魏跃  赵桂华  杨鹤同  朱耀  李玉进  张蜀宁
作者单位:1. 江苏农林职业技术学院,江苏镇江212400;南京农业大学南方蔬菜遗传改良重点开放实验室,南京210095
2. 江苏农林职业技术学院,江苏镇江,212400
3. 南京农业大学南方蔬菜遗传改良重点开放实验室,南京,210095
基金项目:国家自然基金重点项目(30830079); 教育部‘111’项目(B08025)
摘    要:采用常规压片法对羽衣甘蓝花粉母细胞减数分裂及雄配子体发育进行了细胞学观察,结果显示:羽衣甘蓝减数分裂类似甘蓝种,细胞质分裂为同时型,四分体以正四面体型或十字交叉型为主;终变期有9个二价体,此时可进行染色体计数;中期Ⅰ和Ⅱ少数细胞中可见赤道板外染色体,后期Ⅰ和Ⅱ存在落后染色体,四分体时期可观察到少量含微核的异常四分体;单核靠边期时花蕾长度约为2.0~2.2 mm,小孢子经过发育最终成为3-细胞型花粉并具3个萌发孔,成熟花粉中败育花粉比率为1.3%.

关 键 词:羽衣甘蓝  花粉母细胞  减数分裂  雄配子体

Observation of Pollen Mother Cell Meiosis and Male Gametophyte Development in Brassica oleracea L.var.acephala DC.
WEI Yue,ZHAO Gui-hua,YANG He-tong,ZHU Yao,LI Yu-jin,ZHANG Shu-ning.Observation of Pollen Mother Cell Meiosis and Male Gametophyte Development in Brassica oleracea L.var.acephala DC.[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(9):1753-1757.
Authors:WEI Yue  ZHAO Gui-hua  YANG He-tong  ZHU Yao  LI Yu-jin  ZHANG Shu-ning
Institution:WEI Yue1,2,ZHAO Gui-hua1,YANG He-tong1,ZHU Yao1,LI Yu-jin1,ZHANG Shu-ning2(1 Jiangsu Polytechnic College of Agriculture and Forestry,Zhenjiang,Jiangsu 212400,China,2 Key Laboratory of Southern Vegetable Crop Genetics Improvement,Nanjing Agricultural University,Nanjing 210095,China)
Abstract:The chromosome preparation technique was adopted to examine the meiosis process of pollen mother cells and the development of the male gametophytes in kale from the cytological angle.The results indicated that the meiosis process of kale was as the same as the other Brassica species,the pollen mother cells carried on their meiosis and cytokinesis simultaneously and their tetrads were mainly tetrahedral or decussate types;There appeared nine bivalents in diakinesis and chromosome numbers could be counted in ...
Keywords:Brassica oleracea L  var  acephala DC    pollen mother cell  meiosis  male gametophyte  
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