| 拟南芥雄性不育相关基因LDAD1&2的遗传定位(英文) |
| |
| 引用本文： | 周树敏,袁晓君,韦嘉励,张卫.拟南芥雄性不育相关基因LDAD1&2的遗传定位(英文)[J].西北植物学报,2011,31(10). |
| |
| 作者姓名： | 周树敏 袁晓君 韦嘉励 张卫 |
| |
| 作者单位： | 上海大学生命科学学院,上海市能源作物育种及应用重点实验室,上海200444 |
| |
| 基金项目： | 上海市高校选拔优秀青年教师科研专项; 国家自然基金(30870225); 上海市教委分子生理学重点学科 |
| |
| 摘 要： | 利用EMS诱变筛选手段分离到一株拟南芥类似花药不开裂雄性不育突变体(like-defective in anther de-hiscence,ldad),其果荚干瘪,花药不能开裂且花粉败育。遗传分析表明,突变体的表型受2个隐性基因控制;细胞学观察发现,在花药发育过程中伴随着小孢子的降解;通过图位克隆初步对ldad的2个突变位点分别定位,一个定位在1号染色体上SSLP标记F22L4与端粒之间171 kb的区间,另一个定位在5号染色体上SSLP标记T10O8与端粒间150 kb的区间内;生物信息学分析显示此区间内未见育性相关的已知基因。该研究的结果对进一步克隆LDAD1&2基因及探讨其在花药发育中的功能具有重要意义。
|
| 关 键 词： | 图位克隆 拟南芥 雄性不育 小孢子 花药 |
Mapping of Male Sterile Genes LDAD1&2 in Arabidopsis thaliana |
| |
| ZHOU Shu-min,YUAN Xiao-jun,WEI Jia-li,ZHANG Wei.Mapping of Male Sterile Genes LDAD1&2 in Arabidopsis thaliana[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(10). |
| |
| Authors: | ZHOU Shu-min YUAN Xiao-jun WEI Jia-li ZHANG Wei |
| |
| Institution: | ZHOU Shu-min,YUAN Xiao-jun,WEI Jia-li,ZHANG Wei*(College of Life Sciences,Shanghai Key Laboratory of Bio-energy Crops,Shanghai University,Shanghai 200444,China) |
| |
| Abstract: | A male sterile mutant like-defective in anther dehiscence,ldad,was isolated by enthyl-methane sulfonate(EMS) mutagenesis strategy.Genetic analysis indicated the mutation phenotype was controlled by two recessive nuclear genes.The mutant had empty siliques and indehiscent anthers with shrink pollen grains.Cytological observation showed the microspore degradation occurred during anther development.The two mutation loci were mapped to the region of 171 kb between top-telomere and SSLP marker F22L4 on Chr.1,and... |
| |
| Keywords: | map-based cloning Arabidopsis thaliana male sterile microspore anther |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
