甘蓝型油菜花粉离体培养

通过对甘蓝型油菜花粉发育阶段和活力的检测确定花粉发育的时期,分离出单核晚期花粉进行离体培养.结果表明,(1)筛选出适合油菜小孢子花粉离体培养的液体培养基为T_1+怀特维生素(White's vitamins)+2%椰子汁+0.5 mol/L麦芽糖,在此培养基上花粉的成熟率可达25.1%,萌发率达6.3%.(2)筛选出适合成熟花粉离体萌发液体培养基为0.6 mol/L麦芽糖+1.6 mmol/L硼酸+2.9 mmol/L硝酸钙+29.6 μmol/L VB_1,在此培养基上,自然成熟花粉的萌发率可达75.2%.将离体培养成熟的花粉培养在萌发培养基,萌发的花粉占成熟花粉的66.3%.

Maturation of Microspores of Brassica napus L.in vitro

Pollen developmental progress and viability of Brassica napus L.in vivo were investigated for determining pollen development stages.Liquid medium composed of T1+White's vitamins+2% coconut milk+0.5 mol/L maltose was chosen which was the most appropriate medium for in vitro culture of B.napus L.microspores.With this medium,the rates of maturation and germination pollen were 25.1% and 6.3%,respectively.Liquid medium composed of 0.6 mol/L maltose+1.6 mmol/L boracic acid+2.9 mmol/L Ca(NO_3)_2+29.6 μmol/L VB_1 was chosen for germination of matured pollen in vivo.The rate of germination pollen was 75.2%.When pollen matured in vitro was cultured in the germination medium,the rate of germination pollen was 66.3%.