| 草莓高效离体叶片再生体系的建立 |
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| 引用本文： | 王翡,高志红,章镇,乔玉山,王萌,李国平,糜林.草莓高效离体叶片再生体系的建立[J].西北植物学报,2010,30(5). |
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| 作者姓名： | 王翡 高志红 章镇 乔玉山 王萌 李国平 糜林 |
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| 作者单位： | 1. 南京农业大学,园艺学院,南京,210095
2. 江苏省丘陵地区镇江农业科学研究所,江苏句容,212400 |
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| 基金项目： | 江苏省农业资源开发局项目，江苏省科技支撑计划 |
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| 摘 要： | 以草莓'明宝(Meiho)'和'红颊(Benihope)'的叶片为外植体,研究了不同基本培养基、暗培养时间、植物生长调节剂、叶龄、不同放置方式对其不定芽再生的影响.结果表明:各品种叶片不定芽离体再生的最佳条件不同.'明宝'叶片的最佳不定芽再生培养基为MS+2.5 mg/L TDZ+0.1 mg/L IBA+0.1 mg/L 2,4-D,叶片再生的最佳叶龄为30～40 d,再生率可达82.8%;'红颊'叶片的最佳不定芽再生培养基为MS+2.0 mg/L TDZ+0.1 mg/L IBA+0.1 mg/L 2,4-D,叶片再生的最佳叶龄在10～20 d,再生率可达79.8%.2个品种叶片暗培养14 d可以提高不定芽再生率;叶片正放比反放再生效果好;添加8 mg/L AgNO3和1 000 mg/L活性炭可有效提高再生率.
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| 关 键 词： | 草莓 明宝 红颊 不定芽再生 |
Establishment of Regeneration System of Strawberry |
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| WANG Fei,GAO Zhi-hong,ZHANG Zhen,QIAO Yu-shan,WANG Meng,LI Guo-ping,MI Lin.Establishment of Regeneration System of Strawberry[J].Acta Botanica Boreali-Occidentalia Sinica,2010,30(5). |
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| Authors: | WANG Fei GAO Zhi-hong ZHANG Zhen QIAO Yu-shan WANG Meng LI Guo-ping MI Lin |
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| Institution: | WANG Fei1,GAO Zhi-hong1,ZHANG Zhen1,QIAO Yu-shan1,WANG Meng1,LI Guo-ping2,MI Lin2(1 College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China,2 Zhenjiang Agricultural Scientific Institute,Jurong,Jiangsu 212400,China) |
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| Abstract: | Taking the strawberry leaves of 'Meiho' and 'Benihope' as experimental material,we have studied the effects of different basic media,dark culture time,hormone concentration,leaf age and way of placement on inducing adventitious shoot of strawberry.The results showed that regeneration of adventitious shoots of different cultivars have different optimum conditions.The best medium of 'Meiho' was MS+2.5 mg/L TDZ+0.1 mg/L IBA+0.1 mg/L 2,4-D,the optimum leaf age was 30~40 days,the regeneration rate was up to 82.8... |
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| Keywords: | strawberry Meiho Benihope regeneration of adventitious shoots |
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