| 苜蓿组织培养中球形胚发生时特异蛋白质和同工酶分析 |
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| 引用本文： | 刘天磊,江晓雯,等.苜蓿组织培养中球形胚发生时特异蛋白质和同工酶分析[J].西北植物学报,2002,22(3):625-629. |
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| 作者姓名： | 刘天磊 江晓雯 |
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| 作者单位： | 兰州大学生命科学学院,兰州,730000 |
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| 摘 要： | 试验在苜蓿组织培养中，对球形胚形成过程中特异蛋白质表达的模式、过氧化物酶及酯酶同工酶酶谱变化进行研究，结果表明：苜蓿组织培养中从胚性愈伤组织到球形胚发育的进程中，顺序消失和出现了11种中小分子量多肽；过氧化物酶同工酶酶谱发生了显著的变化；酯酶同工酶酶谱变化不大，但其总活力对于维持体细胞胚胎发生是必须的。
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| 关 键 词： | 苜蓿 组织培养 球形胚发生 特异蛋白质 同工酶分析 |
| 文章编号： | 1000-4025(2002)03-0625-05 |
| 修稿时间： | 2000年11月10 |
The analysis of special proteins and isozymes in globular embryogenesis of alfalfa in tissue culture |
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| LIU Tian-lei,JIANG Xiao-wen,WANG Lun-shan.The analysis of special proteins and isozymes in globular embryogenesis of alfalfa in tissue culture[J].Acta Botanica Boreali-Occidentalia Sinica,2002,22(3):625-629. |
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| Authors: | LIU Tian-lei JIANG Xiao-wen WANG Lun-shan |
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| Institution: | LIU Tian-lei,JIANG Xiao-wen,WANG Lun-shan * |
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| Abstract: | The expression patterns of soluble proteins,peroxidase and esterase isozymes were analysed on globular embryogenesis in tissue cultures of alfalfa,it was concluded that from embryogenic callus to globular embryo genesis 11 peptides emerged and disappeared orderly,the patterns of peroxidase isozyme changed dramatically,esterase isozymes changed slightly while the total enzyme activity was important to keeping the potential of somatic embryogenesis. |
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| Keywords: | Medicago sativa protein peroxidase esterase isozyme globular embryo |
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