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4种红景天植物的组织培养研究
引用本文:刘海军,郭斌,晏琼,刘玉军,刘春朝.4种红景天植物的组织培养研究[J].西北植物学报,2006,26(10):2023-2027.
作者姓名:刘海军  郭斌  晏琼  刘玉军  刘春朝
作者单位:1. 中国科学院过程工程研究所,生化工程国家重点实验室,北京,100080;北京林业大学,生物科学与技术学院,北京,100083
2. 中国科学院过程工程研究所,生化工程国家重点实验室,北京,100080;中国科学院研究生院,北京,100039
3. 中国科学院过程工程研究所,生化工程国家重点实验室,北京,100080
4. 北京林业大学,生物科学与技术学院,北京,100083
基金项目:中国科学院与西藏自治区科技合作项目
摘    要:以大花红景天、云南红景天、长鞭红景天和库页红景天的茎和叶为外植体进行组织培养,结果表明:大花红景天以茎为外植体诱导芽效果最好,其它3种红景天以叶为外植体诱导芽效果最好。云南红景天和长鞭红景天适合的芽诱导激素组合是0.1mg/L NAA和2.5mg/L 6-BA的组合,在该激素水平下两种红景天的出芽频率分别达到71%和84%;大花红景天和库叶红景天适合的芽诱导激素组合是0.5mg/L NAA和2.5mg/L 6-BA的组合,在该激素水平下两种红景天的出芽频率均达到80%。长鞭红景天和库叶红景天在添加IBA的培养基上容易生根形成完整植株,生根率分别达到87%和73%;经过炼苗后,长鞭红景天再生苗能够成功移栽,成活率达66%。

关 键 词:红景天  组织培养  再生植株
文章编号:1000-4025(2006)10-2023-05

Tissue Cultures of Four Rhodiola Species
LIU Hai-jun,GUO Bin,YAN Qiong,LIU Yu-jun,LIU Chun-zhao.Tissue Cultures of Four Rhodiola Species[J].Acta Botanica Boreali-Occidentalia Sinica,2006,26(10):2023-2027.
Authors:LIU Hai-jun  GUO Bin  YAN Qiong  LIU Yu-jun  LIU Chun-zhao
Institution:1.National Key Laboratory of Biochemical Engineering, Institute of Process Engineering. Chinese Academy of Sciences, Beijing 100080,China ; 2.College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; 3.Graduate School of the Chinese Academy of Sciences, Beijing 100093,China
Abstract:The stems and leaves of Rhodiola crenulata, Rhodiola yunnanensis, Rhodiola fastigiata and Rhodiola sachalinensis were in vitro cultured as the explants, the stems of Rhodiola crenulata and the leaves of Rhodiola yunnanensis ,Rhodiola fastigiata and Rhodiola sachalinensis were found to be the best parts for shoot induction. The optimal hormone combination for shoot induction of Rhodiola yunnanensis and Rhodiola crenulata was 0.1 mg/L NAA plus 2.5 mg/L 6-BA and the shoot induction percentages of the two species were 71% and 84% with the optimal hormone combination,respectively;The optimal hormone combination for shoot induction of Rhodiola fastigiata and Rhodiola sachalinensis was 0. 5 mg/L NAA plus 2.5 mg/L 6-BA and the shoot induction percentages of the two species reached 80% with the optimal hormone combination;the induced shoots of Rhodiola fastigiata and Rhodiola sachalinensis were easy to root on the medium with IBA added and the rooting percentages were 87% and 73% ,respectively; After having been hardened the regeneration plantlets of Rhodiola fastigiata could be successfully transplanted and survive at the rate of 66 %.
Keywords:Rhodiola  tissue culture  regeneration plantlet
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