影响油菜子叶外植体不定芽高频率再生的因素

采用７个甘蓝型和２个白菜型油菜品种研究了影响子叶外植体芽再生的一些因素和不同基因型的子叶外植体的离子体培养反应和芽再生能力。结果表明，苗龄４ｄ的幼苗叶子含２，４－Ｄ０．５－１．２ｍｇ／Ｌ和６－ＢＡ０．２ｍｇ／Ｌ的培养基培养２或６ｄ后再转到分化培养基上培养，芽再生率３４％－４６％。

FACTORS AFFECTING SHOOT REGENERATION AT HIGH FREQUENCY FROM IN VITRO CULTURE COTYLEDON OF BRASSICA NAPUS AND B.CAMPESTRIS

The cotyledon explants of 7 Brassica napus and 2 B.campestris varieties (lines) were cultured and some of factors affecting shoot regeneration at high frequency were also studies.Results showed that the frequencies of shoot regeneration of cotyledons explants from 4 d old in vitro grown seedlings were markedly increased from 34% to 46% via a preculture procedure of 2 days period using the media containing the hormone combinations of 2,4 D 0.5-1.2 g/L and 6 BA 0.2 mg/L.AgNO 3(6 mg/L) added in regeneration media was advantageous for the formation of adventitions shoots whereas L serine and L glutamine was not good action on shoot formation.Under same culture condition,the shoot regeneration rate of B.napus and B.campestris was 28.85%-90.57% and 24.32%-27.78%,respectively.This indicated that the shoot regeneration capacity of interspecies and inter varieties (lines) was greatly different.