| 绿色荧光蛋白基因TuMv病毒表达载体的构建 |
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| 引用本文： | 陈书霞,王晓武,程智慧.绿色荧光蛋白基因TuMv病毒表达载体的构建[J].西北植物学报,2005,25(7):1395-1398. |
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| 作者姓名： | 陈书霞 王晓武 程智慧 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西杨陵,712100
2. 中国农业科学院,蔬菜花卉研究所,北京,100086 |
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| 基金项目： | 农业部“948”项目(991055)，蔬菜遗传生理重点实验室资助项目 |
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| 摘 要： | 本研究通过设计引物进行PCR扩增得到绿色荧光蛋白GFP基因，将PCR产物酶切以后与芜菁花叶病毒表达载体相连，得到的阳性克隆通过多对引物组合进行PCR验证及序列测定，说明GFP基因已经连接到该病毒表达载体中，而且没有发生碱基误配及错配。该GFP表达载体的构建为进一步利用TuMv的全长cDNA侵染性克隆进行外源基因的转化奠定了基础。
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| 关 键 词： | 绿色荧光蛋白基因 TuMv病毒表达载体 构建 |
| 文章编号： | 1000-4025(2005)07-1395-04 |
Construction of TuMv Virus-expressing Vector of Green-fluorescent Protein Gene |
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| CHEN Shu-xia,WANG Xiao-wu,CHENG Zhi-hui.Construction of TuMv Virus-expressing Vector of Green-fluorescent Protein Gene[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(7):1395-1398. |
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| Authors: | CHEN Shu-xia WANG Xiao-wu CHENG Zhi-hui |
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| Institution: | CHEN Shu-xia~1,WANG Xiao-wu~2,CHENG Zhi-hui~1 |
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| Abstract: | The study conducted PCR amplification of the designed primers to obtain green-fluorescent protein gene,and the PCR products,after being enzyme digested,were connected with TuMv virus-expressing vector to produce the positive clones for which PCR identification and sequencing were carried out with several pairs of primer combinations to prove green-fluorescent protein gene had been connected to the vector without base-pairing errors.The construction of the vector would provide the basis for further making full use of full-length TuMv cDNA infectivity clones to transform exogenous genes. |
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| Keywords: | green-fluorescent protein gene TuMv virus-expressing vector construction |
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