BT型细胞质雄性不育水稻及其三系的线粒体DNA研究

用RAPD技术对BT型水稻胞质雄性不育系秀A及其保持系秀B、恢复系湘晴以及杂种F1代的线粒体DNA进行了比较分析。结果表明不育系与其保持系间存在显著差异；不育系与其F1之间mtDNA也存在差异。在引物OPJ－08的扩增产物中，秀A扩增出一条分子量为800bp的多态性片段，在引物OPK－10的扩增产物中，杂种F1扩增出一条分子量为900bp的片段。把这两片段回收、克隆并制备探针，OPJ－08800的Southern杂交结果显示不育系与其F1杂交图谱存在多态性；OPK－10900的Suthern杂交结果显示不育系与其保持系同存在差异。推测这两片段与育性可能有一定的联系。

RAPD analysis for mitochondrial DNAs of the BT cytoplasmic male sterile (cms) line and its associated lines in rice (Oryza sativa)

BT type rice cytoplasmic male sterility line were compared with its maintainer,restorer and F 1 hybrid using mitochondrial DNA (mtDNA) RAPD analysis.The results showed that difference was between not only the cms line and its maintainer,but also cms line and its F 1 hybrid.Primer OPJ-08 generated a polymorphic product of 800 bp in sterile line,and primer OPK-10 amplified a specific fragment of 900 bp in F 1 hybrid.The two fragments were then cloned into plasmid vector and converted to RFLP probes.Southern hybridization patterns revealed polymorphism between sterile line and F 1 hybrid using OPJ-08 800 fragment as probe,and between sterile line and maintainer line using OPK-10 900 fragment as probe.We infered that the two mtDNA fragments may be associated with cms in rice.