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烟草大小孢子发生和雌雄配子体发育研究
引用本文:廖菊够,康洪梅,代瑾然,姚 恒,马文广,陈穗云.烟草大小孢子发生和雌雄配子体发育研究[J].西北植物学报,2013,33(1):11-16.
作者姓名:廖菊够  康洪梅  代瑾然  姚 恒  马文广  陈穗云
作者单位:1. 云南大学生命科学学院植物学系,昆明,650091
2. 云南省烟草农业科学研究院,云南玉溪,653100
基金项目:云南省烟草公司项目(2010YN09,2011YN60,2011174);云南大学生命科学学院植物科学研究所青年教师基金项目(ZW201002)
摘    要:采用卡宝品红染色压片和石蜡切片法对迪勃纳氏烟草(Nicotiana debneyi)大小孢子发生和雌雄配子体发育过程进行了研究.结果发现:(1)迪勃纳氏烟草为两性花,其小孢子发生和雄配子体发育早于大孢子.(2)迪勃纳氏烟草小孢子发生和发育过程基本正常,减数分裂过程有少数细胞出现滞后染色体、染色体断片和染色体桥等异常现象;其胞质分裂为同时型,四分体为十字交叉型和正四面体型,成熟的花粉粒为2细胞型.(3)迪勃纳氏烟草为2室子房,中轴胎座,倒生胚珠,胚珠多数,胚囊发育为蓼型,大孢子发生和发育过程未观察到异常现象.

关 键 词:迪勃纳氏烟草  大孢子发生  小孢子发生  雌配子体  雄配子体

Megasporogenesis,Microsporogenesis and Development of Female and Male Gametophyte of Nicotiana debneyi
LIAO Jugou,KANG Hongmei,DAI Jinran,YAO Heng,MA Wenguang,CHEN Suiyun.Megasporogenesis,Microsporogenesis and Development of Female and Male Gametophyte of Nicotiana debneyi[J].Acta Botanica Boreali-Occidentalia Sinica,2013,33(1):11-16.
Authors:LIAO Jugou  KANG Hongmei  DAI Jinran  YAO Heng  MA Wenguang  CHEN Suiyun
Institution:1(1 School of Life Science,Yunnan University,Kunming 650091,China;2 Tobacco Research Institute,Yuxi,Yunnan 653100,China)
Abstract:The megasporogenesis,microsporogenesis and the development of female and male gametophyte of Nicotiana debneyi were studied by carbol fuchsin staining and paraffin sectioning.The results showed that:(1)N.debneyi is hermaphrodite flower and the microspore develops earlier than megaspore.(2)Microsporogenesis and development of male gametophyte of N.debneyi are normal but there are chromosome bridges,chromosome fragment,laggard chromosome in a few cells.The meiosis in microspore of N.debneyi is simultaneous cytokinesis and the tetards are decussate or tetrahedral types.The mature pollens belong to 2 cell type.(3)N.debneyi is ovary 2-locular,axile placenta,ovules numerous and polygonum type of mature embryo sac.Megasporogenesis and the development of female gametophyte of N.debneyi are normal.
Keywords:Nicotiana debneyi  megasporogenesis  microsporogenesis  female gametophyte  male gametophyte
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