小麦耐盐细胞系及后代耐盐稳定性的生化分析

以离体培养筛选出的耐盐小麦变异系及其后代为材料，对其耐盐稳定性进行有关生理生化特性分析。结果表明：（１）随着耐盐愈伤组织耐盐能力的提高，过氧化物同工酶酶带谱加，与对照相比，三个区域出现了明显差异；（２）从耐盐愈伤组织酯酶同工酶酶谱发现，耐盐愈伤组织具有Ｃ２，Ｃ５，Ｃ９，Ｃ１１新增加酶带；（３）耐盐系后代幼苗能维持较高的Ｋ＾＋／Ｎａ＾＋比值，在盐浓度为０．９％时，其Ｋ＾＋／Ｎａ＾＋为０．７８８，而对

Biochemical analysis of salt tolerant cell line and their progeny of wheat

Salt tolerant wheat variety that selecting under salt stress in vitro ,and their progeny were studied on genetic stability by biochemical analysis.Results showed that:(1) There were 3 mainly changes of POD and 4 additional bands of estease in salt tolerant cell line comparing with CK;(2) The seedling of salt tolerant lines showed that ability maintain higher K +/Na + rate than the CK.Their rate of K +/Na + was 0.788 in salt tolerent line,but CK only 0.454 in 0.9% NaCl concentration;(3) The content of Pro in seedling of progeny of regenerated plant of salt tolerant line can be 6.93 times in 1.2% NaCl concentration;(4) Two additional bands of gliadin were be found in the seeds of salt tolerant wheat line;Characters of salt tolerance in wheat were discussed.