| 29个香蕉基因型遗传多样性的SRAP分析 |
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| 引用本文： | 谢子四,刘德兵,魏守兴,陈业渊,谢建吉,魏军亚.29个香蕉基因型遗传多样性的SRAP分析[J].西北植物学报,2012,32(8):1547-1552. |
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| 作者姓名： | 谢子四 刘德兵 魏守兴 陈业渊 谢建吉 魏军亚 |
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| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所,农业部热带作物种质资源利用重点开放实验室,海南儋州571737
2. 2海南大学 应用科技学院(儋州校区),海南儋州,571737 |
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| 基金项目： | 海南省自然科学基金（30823）;中央级公益性科研院所基本科研业务费专项基金（PZS085）;农业部热带作物种质资源利用重点开放实验室开放基金（KFKT201104）;现代产业体系香蕉综合试验站站长经费（CARS3216）;物种资源保护项目(12RZZY19) |
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| 摘 要： | 采用SRAP分子标记技术对29个香蕉品种(系)的多样性进行研究,结果显示,64对SRAP引物中筛选出25个多态性较高的引物组合,共扩增出324条条带;UPGAM聚类图显示所有供试的29个香蕉品种(系)可分为2个类群且与基因型相一致;实验结果与形态、农艺性状标记分类基本一致。研究表明,SRAP技术可有效运用于香蕉基因型的遗传和育种研究。
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| 关 键 词： | 香蕉 SRAP 遗传多样性 |
Analysis of Genetic Diversity of 29 Banana Genotypes by SRAP |
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| XIE Zi si,LIU De bing,WEI Shou xing,CHEN Ye yuan,XIE Jian ji,WEI Jun ya.Analysis of Genetic Diversity of 29 Banana Genotypes by SRAP[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(8):1547-1552. |
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| Authors: | XIE Zi si LIU De bing WEI Shou xing CHEN Ye yuan XIE Jian ji WEI Jun ya |
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| Institution: | 1(1Germplasm Research Institute of Tropical Crops of Chinese Academy of Tropical Agriculture Sciences,Key Tropical Crops Germplasm Utilization,Ministry of Agriculture of China,Danzhou,Hainan 571737,China;2Applied Science and Technology College,Hainan University,Danzhou,Hainan 571737,China) |
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| Abstract: | In this experiment,a molecular marker sequence related amplified polymorphism (SRAP) was applied to determine the diversity and relationships among 29 banana (Musa spp.) cultivars.The results showed that 25 out of 64 primer combinations turned out to be polymorphic and 324 polymorphism bands were obtained.The genetic relationships were analyzed using unweighted pair group method of arithmetic average cluster analysis (UPGMA),and the genetic similarity coefficients were calculated among the 29 banana cultivars.The genetic relationship among cultivars was correlated to the origin region of cultivars,and all 29 cultivars could be clustered into two major clusters closely corresponded with the genome composition.The result was consistent with the morphology,agronomic trait classification.All results indicated that the amplification of SRAP markers is an efficient method for estimating genetic similarity among banana cultivars and providing a scientific basis for banana genetic and breeding research. |
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| Keywords: | Musa spp SRAP genetic diversity |
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