蓖麻杂交种的SSR鉴定及遗传变异分析

采用SSR标记对蓖麻CSR24×CSR181杂交所得的F1种子进行分析,为蓖麻早期杂种鉴定和遗传变异分析奠定技术与理论基础。结果表明:(1)各位点鉴定所得结果基本一致,除RCM207和RC129位点鉴定的杂种率未超过10%外,其它位点的杂种率都十分接近,在13.46%～17.27%之间。(2)少数个体在相关位点发生了变异,在引物RC242的扩增图谱中有4个单株出现了双亲特异条带的缺失,产生了双亲都没有的新条带;在引物Rco23、Rco26、Rco29、RC129、RCM613和RCM999的扩增结果中出现了父本特异条带的缺失,同时产生了一条新条带。(3)多样性及UPGMA聚类分析表明杂交后代遗传变异显著,子代个体与亲本间的遗传相似系数在0.45～1.0之间,个体间差异明显。

Identification and Genetic Variation Analysis of Castor Bean Hybrid by SSR

In order to identify the hybrid purity of castor bean in early seedling stage or before sowing,castor bean hybrid verification and genetic diversity of F₁ seeds derived from CSR24×CSR181 hybridization were performed by SSR markers.It was showed that the results by different loci were basically consistent,and the hybrid rates estimated by most loci were from 13.46% to 17.27%,only which evaluated by RCM207 and RC129 were less than 10%.The variation was occurred on some loci of several individuals,4 progenies had non parent markers and generated two new bands in primer RC242;the paternal special bands were absent and a new band was appeared in some progenies in primer Rco23,Rco26,Rco29,RC129,RCM613 and RCM999.The genetic diversity and cluster analysis of SSR data indicated that the distinct differentiation existed in F₁ progenies,the genetic variation was obvious,and genetic similarity coefficient between parents and progenies was 0.45~1.0.It was provided a method for early verification of true hybrids and offered the theoretical reference for genetic analysis of offsprings.