辣椒雄性不育材料小孢子发生的细胞形态学观察

用石蜡切片技术,在光学显微镜下观察了辣椒雄性不育材料1A及其保持系1B的小孢子发育过程和各时期的形态特征.结果表明,雄性不育材料1A的小孢子败育发生在四分体至单核花粉粒时期,此时绒毡层细胞异常肥大,四分体受到挤压后破裂并降解,无法形成正常的单核花粉粒.扫描电镜观察结果表明,保持系1B的花粉粒结构完整,表面有3个明显的萌发沟;而雄性不育材料1A的成熟花粉粒形状不规则,空瘪,有部分花粉粒解体,败育比较彻底,说明该雄性不育材料在辣椒育种工作中有较高的利用价值.

Cyto-morphological Observation of the Microsporogenesis of Male Sterility Materials in Pepper (Capsicum annuum L. )

Paraffin sectioning was employed to observe the microsporogenesis of male sterility pepper line 1A and its maintainer 1B and their morphological features at different microsporogenesis stages with light microscope.It was shown that in male sterility line 1A microspore abortion occurred from the tetrad stage to the uninucleate pollen grain stage and during this period tapetum cells were hypertrophy so that tetrads were extruded to crack and break down so that they could not form normal uninucleate pollen grains.Scanning electron microscope observation indicated that maintainer 1B had structurally complete pollen grains,which had obvious germination furrows;the pollen grains of male sterility line 1A were irregular in shape,hollow and wrinkled,and some of them broke down and completely aborted,which indicated the line was of high value in pepper breeding.