杯山药零余子愈伤组织诱导及植株再生的研究

对怀山药（Ｄｉｏｓｃｏｒｅａ ｏｐｐｏｓｉｔａ）零余子愈伤组织的诱导、分化、再生苗的生根和移栽进行了研究。结果表明：⑴在不同激素组合的培养基上怀山药零余子均能产生愈作组织，而且具有一次成苗的能力。ＢＡ２ｍｇ／Ｌ＋ＮＡＡ２ｍｇ／Ｌ的培养基对诱导愈伤组织最有利，其出愈率达１００％；⑵在愈伤组织的分化中，ＢＡ１ｍｇ／Ｌ＋ＮＡＡ１ｍｇ／Ｌ的激素组合是最佳的，其分化率为６３．６％，且多形成丛生芽；⑶再生植株

Study on the callus induction and plantlet regeneration from the bulbil of Dioscorea opposita

This paper deals with studies on \%in vitro\% culture of bulbil of Dioscorea opposita ,including callus induction and differentiation,plantlet root development and transplantation.Results showed:(1) Calli were obtained from bulbil on media containing different kinds of auxin and cytokinin,also the plantlets could be developed at the same time.It was advantageous to callus induction on MS basal medium supplemented with BA 2 mg/L and NAA 2 mg/L.The frequency of callus induction was 100%;(2) The combination of BA 1 mg/L and NAA 1 mg/L was the best to callus differentiation of which ratio was 63.6%.Most of calli formed multiple buds;(3) 91.2% of the regenerated plantlets treated with KT 2 mg/L+NAA 0.02 mg/L+PP333 0.1 mg/L could develop their roots,grew robustly and robusted green leaves;after the regenerated plantlets were transplanted to soil,they grew robustly and robusted green leaves,moreover could adapt to enviroment.