不同地域乌拉尔甘草基因组的FISH分析与染色体识别

在核型分析与染色体识别基础上,分别以番茄45S和5S rDNA为探针,对3种不同地域的乌拉尔甘草进行FISH分析.结果表明:内蒙古鄂托克前旗的乌拉尔甘草核型公式为2n=2x=16=6m+10sm (2SAT),新疆阿勒泰地区的乌拉尔甘草核型公式为2n=2x=16=4m+12sm(2SAT),内蒙古喀喇沁旗乌拉尔甘草核型公式为2n=2x=16=4m+12sm(2SAT);其第8染色体均带有随体.3种乌拉尔甘草基因组内均有1对5S rDNA和1对45S rDNA杂交位点.核型分析显示,5S rDNA杂交位点均位于第2染色体的短臂部位,45S rDNA杂交位点均位于第8染色体的次缢痕和随体部位.45S与5S rDNA在3种乌拉尔甘草中期分裂相上的位点数和分布情况高度一致,表明来自3种不同地域的乌拉尔甘草在染色体结构水平上没有较大的分化.

FISH Analysis and Chromosome Identification of Glycyrrhiza uralensis Genome from Different Territory

On the basis of the karyotype analysis and chromosome identification,both 45S and 5S rDNA used as probes were hybridized to numerous chromosomes and interphase nucleus of Glycyrrhiza uralensis from three different territory (Inner Mongolia Kalaqin area,Inner Mongolia Etuoke area,Sinkiang Aletai area) respectively.The FISH results showed that the karyotype of G.uralensis obtained from Inner Mongolia Etuoke area was 2n=2x=16=6m+10sm(2SAT),that of G.uralensis obtained from Sinkiang Aletai area was 2n=2x=16=4m+12sm(2SAT),and that of G.uralensis obtained from Inner Mongolia Kalaqin area was 2n=2x=16=4m+12sm(2SAT).The chromosome 8 has satellites.A pair of 5S rDNA signals,which were located on the regions of short arms of chromosome 2,were detected in the genome of G.uralensis from all three different territories,also exhibited a pair of 45S rDNA signals which were located on the regions of satellite and secondary constriction of chromosome 8.The number and location of 45S and 5S rDNA signals are the same,so it indicates that there is no greater differentiation in the level of the chromosome structure of G.uralensis from the three different territories.