曼陀罗叶水浸液对大豆幼根生长及细胞分裂的影响

以曼陀罗叶干粉为供体,大豆种子为受体,用室内培养皿法研究了曼陀罗叶水浸液化感胁迫对大豆种子萌发、幼根生长、根毛发育和根尖细胞分裂的影响。结果表明:各试验浓度的曼陀罗叶干粉水浸提液均抑制了大豆种子的萌发以及幼根和侧根的生长,且幼根生长随胁迫浓度的增加呈现逐渐缩短变粗变褐的趋势;化感胁迫还使根尖分生区细胞的染色体畸变和微核高频率发生。根毛发生和根尖细胞的分裂对化感胁迫都表现出"低促高抑"的响应,即轻度胁迫能显著促进根毛的生长并提高根尖细胞的分裂指数,而高度胁迫对根毛的生长有极显著的抑制作用,且使根尖细胞的分裂指数显著下降。

Young Root Growth and Cell Division of Soybean Stressed by Aqueous Leachate of Datura stramonium Leaves

Taking the dry powder of Datura stramonium L.leaves as the donor,the soybean seed as the acceptor,we studied the effects of allelochemical stress produced by aqueous leachate on seed germination,young root growth,root hair development,root-tip cell division of soybean by the indoor petri dish with filter paper.The result indicated:In the experiments concentrations scope,the soybean seed germination,young root growth and lateral root growth were inhibited with the dry powder aqueous leachate of D.stramonium L.leaves.And the young root gradually shortened and coarsened and appeared brown spot with the increased stress concentration.In addition,the high-frequency chromosome aberration and micronucleus were induced by allelochemical stress.But the root hair development and root-tip cell division showed a response that they were promoted when lower concentration and inhibited when higher concentration,to wit,the relative lower concentration significantly promoted root hair development and increased the mitotic index while inhibitory effects were observed at higher concentrations of allelochemical stress,the root-tip cell mitotic index obviously to drop.