玉米ST和ATPS部分cDNA序列克隆及分析

硫酸盐转运蛋白(ST)和ATP硫酸化酶(ATPS)是根系吸收硫酸盐和植物体内硫酸盐同化过程的关键蛋白和酶,在硫酸盐的生物转运过程中具有重要作用.以水培玉米农大108根系为材料,并根据已报道的玉米的硫酸盐转运蛋白和ATP硫酸化酶基因保守序列分别设计PCR引物对,采用RT-PCR方法克隆到783 bp和820 bp的部分硫酸盐转运蛋白和ATP硫酸化酶cDNA片段,分别命名为ST_ND108和ATPS_ND108.序列分析和比对结果显示,ST_ND108与已报道的玉米和水稻的高亲和型硫酸盐转运蛋白基因同源性分别为99%和85%;而ATPS_ND108与已报道的玉米ATP硫酸化酶基因同源性达到97%,进化树聚类分析和预测氨基酸的BLAST结果证实ST_ND108为高亲和性硫酸盐转运蛋白基因片段,ATPS_ND108为质体ATP硫酸化酶基因片段.

Cloning and Sequence Analysis of Partial cDNAs of ST and ATPS from Maize

Sulfate transporter and ATP sulfurylase play major role on bio-transportation of sulfate respectively as the key protein and enzyme in the uptake of sulfate by roots and the assimilation of sulfate in plants.Primers were designed according to reported conserve sequences of sulfate transporter(ST)and ATP sulfurylase(ATPS) genes of Zea mays.Using RT-PCR,two fragments of 783 bp and 820 bp were obtained respectively,named as ST_ND108 and ATPS_ND108 from 'Nongda 108' maize roots.Sequence analysis indicated that ST_ND108 was similar to those of the corresponding gene fragments of Zea mays and rice(Oryza sativa) with the homologies standing at 99% and 85%;while,of ATPS_ND108 was 97% similar to Zea mays corresponding gene fragment.Based on the results of phylogenetic tree and alignment of predicted amino sequence,we concluded that ST_ND108 is fragment of high affinity sulfate transporter gene and ATPS_ND108 is fragment of plastid ATP sulfurylase gene.