小麦双引物RAPD分析方法的研究

ＲＡＰＤ标记是近几年迅速发展的一种新型分子标记，标准的ＲＡＰＤ的反应是以１０个寡聚核苷酸作引物，通过ＰＣＲ反应扩增出基因组的部分片段，我们在研究外源ＤＮＡ导入小麦后外源遗传物质的追踪时，对这个方法进行了改进，采取了双引物进行扩增，结果双引物反应能够比单引物反应扩增出更多的多态性片段。分子杂交结果表明，双引物扩增出的新片段与单引物扩增片段无同源性，并对双引物扩增出的多态性片段产生的可能原因进行讨论。

STUDIES ON THE METHOD FOR WHEAT RAPD ANALYSIS BY TWO PRIMER REACTION

Random amplified polymorphic DNA (RAPD) analysis is a new developing technique of molecular marker.The standard RAPD procedure uses a sigle 10 base long random oligonucleotide as a primer to amplify part fragements of the genome by PCR.We modified the procedure by using two primer in tracing foreign gene fragements after introducing foreign DNA into wheat.The result indicated that the two primer RAPD can amplify more fragements than the stardard RAPD technique.Southern analysis revealed that the new bands amplified in two primer reactions had no homology to the bands amplified in single primer reactions involving the same primer.Furthermore,It is discussed that the origin of new RAPD markers may be generated by amplification with the two primer reactions.