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| UV-B辐射对烟草光合色素和几种酶的影响 | |
| 引用本文： | 刘敏,李荣贵,范海,杜桂彩.UV-B辐射对烟草光合色素和几种酶的影响[J].西北植物学报,2007,27(2):291-296. |
| 作者姓名： | 刘敏 李荣贵 范海 杜桂彩 |
| 作者单位： | 1. 青岛大学,山东省天然色素重点实验室,山东青岛,266071;山东师范大学,逆境植物研究所,济南,250014 2. 青岛大学,山东省天然色素重点实验室,山东青岛,266071 3. 山东师范大学,逆境植物研究所,济南,250014 |
| 基金项目： | 山东省青岛市自然科学基金;山东省优秀中青年科学家科研奖励基金 |
| 摘 要： | 对UV-B胁迫条件下烟草叶片相关生理生化指标的变化进行研究.结果表明,UV-B辐照后,烟草叶片内光合色素(叶绿素a、叶绿素b、类胡萝卜素)的含量均呈上升趋势,3种色素比对照分别上升了21%、10%、27%;可溶性蛋白含量呈先上升后下降趋势;过氧化物酶活性明显上升,同工酶图谱分析证实,有一种新的过氧化物酶同工酶表达;利用重组噬夏孢欧文氏菌(Erwinia uredovora)GGPP合成酶免疫家兔,制备了兔抗GGPP合成酶的抗血清.Western blot检测显示,UV-B辐照可使烟草叶片GGPP合成酶(GGPS)的表达量显著提高. |
| 关 键 词： | 烟草 光合色素 蛋白 过氧化物酶 GGPP合成酶 |
| 文章编号： | 1000-4025（2007）02-0291-06 |
| 修稿时间： | 2006-10-17 |
Effects of Enhanced UV-B Radiation on Photosynthetic Pigments and Some Enzymes in Tobacco |
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| LIU Min,LI Rong-gui,FAN Hai,DU Gui-cai.Effects of Enhanced UV-B Radiation on Photosynthetic Pigments and Some Enzymes in Tobacco[J].Acta Botanica Boreali-Occidentalia Sinica,2007,27(2):291-296. | |
| Authors: | LIU Min LI Rong-gui FAN Hai DU Gui-cai |
| Institution: | 1 Key Laboratory for Natural Pigments of Shandong Province, Qingdao University, Qingdao, Shandong 266071, Chinas2 Institute of Plant Stress,Shandong Normal University,Jinan 250014,China |
| Abstract: | Effects of enhanced UV-B radiation on physiological and biochemical characters of tobacco were investigated.The results showed that the photosynthetic pigments(chlorophyll a,chlorophyll b and carotenoids) increased clearly in tobacco leaves under enhanced UV-B radiation compared with which in leaves growing under natural light.The three pigments increased 21%,10% and 27%,respectively.The content of soluble protein increased first and decreased later.The activity of peroxidase increased distinctly at the same time,and a new peroxidase isozyme appeared through analyzing the electrophoresis diagram of peroxidase isozymes.The expression quantity of GGPs increased significantly through Western blot analysis. |
| Keywords: | UV-B |
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