静电场处理对垂枝樱花插穗生根及相关生理生化特性的影响

以垂枝樱花为材料,设置1.5、3.0、4.5、6.0kV/cm共4种场强的静电场处理,分析静电场对垂枝樱花插穗生根性状及相关生理、生化特性的影响,探讨静电场促进垂枝樱花插穗生根的机制。结果显示:(1)1.5～4.5kV/cm场强的静电处理能显著提高插穗的可溶性蛋白、可溶性糖含量,且4.5kV/cm处理的增幅最大,峰值时比对照分别增加43%和44.1%;(2)1.5～4.5kV/cm场强的静电场处理能显著提高插穗的过氧化物酶(POD)活性,降低吲哚乙酸氧化酶(IAAO)活性,且4.5kV/cm处理的效果最明显,与对照相比增幅和降幅最大时分别达到58.9%和17.5%;(3)3.0、4.5kV/cm的静电场处理均能够显著提高插穗的平均单株生根数、根系平均长度、单株根鲜重、根系活力以及插穗成活率,且4.5kV/cm处理的增幅最大,与对照相比分别增加了127.4%、29.2%、88.9%、107.1%、116.3%。研究表明,静电场处理可以有效调节垂枝樱花插穗的可溶性蛋白、可溶性糖的含量以及POD、IAAO的活性,能显著提高插穗的扦插成活率,其最佳静电场场强为4.5kV/cm。

Effects of Electrostatic Field on Rooting and Related Physiological and Biochemical Functions of Cerasus subhirtella var.pendula Cuttings

To improve rooting rate of Cerasus subhirtella var.pendula and study the mechanism of promoting rooting by electrostatic field,four treatments with 1.5,3.0,4.5,6.0 kV/cm of electrostatic field were set.The effects of different treatments of electrostatic field on rooting,functions of physiology and biochemistry of C.subhirtella var.pendula cuttings were studied.The results showed as follows:(1)The contents of soluble protein and soluble sugar in cuttings were significantly increased by electrostatic field with 1.5~4.5 kV/cm.The most effective treatment was 4.5 kV/cm,the largest growth reached 43% and 44.1% compared with the CK,respectively.(2)Enzyme activities of peroxidase(POD) and indoleacetic acid oxidase(IAAO) in cuttings were significantly increased and decreased by electrostatic field with 1.5~4.5 kV/cm,respectively.The most effective treatment was 4.5 kV/cm,the largest growth and reduction reached 58.9% and 17.5% compared with the ck,accordingly.(3)The number of root per plant,length of roots,fresh weight of root,root activity,cuttings survival rate were significantly increased by electrostatic field with 3.0 and 4.5 kV/cm.The most effective treatment was 4.5 kV/cm and the growth rates reached 127.4%,29.2%,88.9%,107.1%,116.3% compared with the CK,respectively.The above results show that contents of soluble protein,soluble sugar and the enzyme activities of the POD and IAAO in cutting of C.subhirtella var.pendula can be effectively regulated by suitable electrostatic field,and the cutting survival rate can be significantly improved.The optimal intensity of electrostatic field is 4.5 kV/cm.