冬枣×宁梨巨枣的子代SRAP鉴定及遗传多样性分析

应用SRAP分子标记方法对冬枣×宁梨巨枣的子代进行了分子鉴定及遗传多样性分析。采用构建基因池的方法对SRAP分子标记引物进行筛选,从88对引物中筛选出15对多态性好、主带清晰的引物,并对子代进行了真实性鉴定及多态性分析。结果表明:(1)15对引物共产生95个多态性条带,平均每对引物产生6.3个多态性条带,显示了较高的多态性比率。(2)80个子代中44个具有父本特征带,鉴定为真杂种。子代遗传多样性及UPGMA聚类分析表明,子代个体与亲本间的遗传相似系数在0.55~0.98之间,个体差异明显。该研究结果为枣树杂交育种提供了重要的分子证据。

Identification and Genetic Diversity Analysis of Hybridprogeny of Ziziphus jujuba Mill.cv.Dongzao and Ziziphus jujuba Mill.cv.Ninglijuzao by SRAP Molecular Markers

The sequence-related amplified polymorphism(SRAP) were used to analyze 80 Ziziphus jujuba Mill.hybrid individuals and their parental lines for detecting true or false hybrids and their genetic variation.Fifteen pairs of primers that generated clear,reproducible and polymorphic bands were selected from 88 pairs of SRAP primers by constructing a gene pool.The results showed that:(1)There are 95 polymorphic bands produced by fifteen primer pairs and the average polymorphic bands was 6.3 per primer pair with a high rate of polymorphism.(2)44 truehybrids were identified among 80 hybrids with paternal characteristics.Diversity and UPGMA cluster analysis showed significant genetic variation of hybrids.The genetic similarity coefficients between individual and parental are from 0.55 to 0.98.The results provided an important molecular data for crossbreeding in Ziziphus jujuba Mill..