中国30个陆地棉主栽品种的SSR指纹图谱

利用SSR分子标记技术,对中国不同生态棉区曾经或正在种植的主要来源于岱字棉、斯字棉、福字棉、乌干达棉的30个陆地棉主栽品种进行了DNA指纹分析。从1 803对SSR引物中筛选到重复性好、多态性丰富的20对核心引物。这些引物分属棉花15条染色体,共检测到116个等位基因,平均每对引物5.8个;PIC值范围为0.384~0.900,平均为0.716;MI值范围为1.152~9.000,平均为4.374。30个品种中有4个品种具有各自的特异引物,可将其与其它26个品种区分开,其它26个品种可利用至少2对引物组合进行区分。为更方便、准确地鉴定各品种,构建了30个品种20对核心引物的十进制数字指纹代码。该研究为陆地棉的品种鉴定和纯度检测、新品种权益保护以及标准DNA指纹库构建提供了重要依据。

SSR Core Primers Reveal DNA Fingerprinting of 30 Upland Cotton (Gossypium hirsutum L.) Varieties in China

DNA fingerprintings of 30 main Upland cotton (Gossypium hirsutum L.) varieties were analyzed using SSR core primers.These experimental materials mainly derived from Deltapine,Stoneville,Foster and Uganda pedigrees,and they have been or are being planted in different ecological cotton-growing areas in China.Twenty pairs of 1 803 primers were confirmed as core primers with stable repeatability and rich polymorphism.These primers belonged to 15 cotton chromosomes,and amplified a total of 116 alleles with an average of 5.8.Polymorphism information content (PIC) values ranged from 0.384 to 0.900 with an average of 0.716,and marker index (MI) values ranged from 1.152 to 9.000 with an average of 4.374.Four of the 30 varieties had their specific primers,by which each variety could be distinguished from the other varieties;each of the other 26 varieties could be distinguished from the other varieties by using at least 2 pairs of primer combinations.In order to identify the 30 varieties more conveniently and accurately,their decimal digital fingerprinting codes were also constructed based on their electrophoresis patterns of the 20 core primers.This study provides important references for identification and purity detection of cotton variety,protection of intellectual property rights and standard database construction of DNA fingerprinting of Upland cotton.