一种快速提取小麦叶片总RNA的方法

从植物组织中提取高质量的RNA是进行植物分子生物学研究的必要前提和关键.同种植物不同器官的组织由于组成分的差异,提取RNA的方法也存在不同的难点.在苯酚法和氯化锂沉淀法的基础上,改进并提出了一种适合小麦叶片总RNA的快速提取方法,消除了蛋白质、DNA、多糖、多酚等污染.该方法提取的小麦叶片总RNA,完整性好、纯度高,可用于RT-PCR、N orthern杂交、RACE等实验操作,而且简单经济、快速、实验结果稳定,重复性好,还适合富含多糖和脂质的植物组织总RNA的提取.

A Method of Quickly Extracting Total RNA from Wheat Leaves

Extracting high-quality RNA from plant tissue is the prerequisite and key in carrying out researches in molecular biology.Because different tissues of same plant species differ in component composition,RNA extraction methods have different nodi.A method of quickly extracting total RNA from wheat leaves were put forward on the basis of phenol extraction method and lithium chloride precipitation extraction method,and it could avoid protein,DNA,polysaccharide and polyphenol contaminations.The total RNA of wheat leaves extracted by this method has good completeness and high purity,and is suitable for RT-PCR,northern blotting and RAGE.This method is simple,economic,quick and of stable performance and good repeatability as well as suitable for extracting total RNA of plant tissues rich in polysaccharides and lipids.