| 重唇石斛传粉生物学与显微动态研究 |
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| 引用本文： | 李振坚,亢秀萍,王雁,于耀,缪崑.重唇石斛传粉生物学与显微动态研究[J].西北植物学报,2009,29(9). |
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| 作者姓名： | 李振坚 亢秀萍 王雁 于耀 缪崑 |
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| 作者单位： | 1. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091
2. 山西农业大学,园艺学院,山西太谷,030801 |
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| 基金项目： | 中央级公益性科研院所专项基金，"948"国家林业局引进项目 |
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| 摘 要： | 通过杂交和自交实验,对重唇石斛授粉亲和力和结实性进行分析,并对其开花期间花粉及蕊柱结构的动态变化进行显微观察,以探讨重唇石斛的最佳杂交授粉时间.结果表明:(1)花器表面结构差异较大,唇瓣、药帽、萼片表皮布满突起、钩刺等,花瓣表皮较平滑;(2)杂交时以重唇石斛为父本或母本均具有选择性,但作为父本亲和力强,杂交结实率可达100%,作为母本杂交结实率最高为60%;自交结实率非常低,为4.3%;(3)花粉培养的最佳配方为30%蔗糖+0.15 mg·L-1 CaCl2;(4)花开第4天后花粉发育饱满,第5～12天花粉发芽率稳定大于70%,蕊柱的乳突细胞于开花第6～10 天时发育饱满.研究表明,重唇石斛花开第5～12 天为最佳授粉时间.
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| 关 键 词： | 重唇石斛 蕊柱 授粉 杂交 结实率 |
Studies on the Pollination Biology and Microscopic Structure of Dendrobium hercoglossum Rchb.f.(Orchidaceae) |
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| LI Zhen-jian,KANG Xiu-ping,WANG Yan,YU Yao,MIAO Kun.Studies on the Pollination Biology and Microscopic Structure of Dendrobium hercoglossum Rchb.f.(Orchidaceae)[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(9). |
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| Authors: | LI Zhen-jian KANG Xiu-ping WANG Yan YU Yao MIAO Kun |
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| Institution: | LI Zhen-jian1,KANG Xiu-ping2,WANG Yan1,YU Yao2,MIAO Kun1(1 Key Lab of Tree Breeding , Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China,2 College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801,China) |
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| Abstract: | To investigate the best pollination date during hybridization of Dendrobium hercoglossum Rchb.f.,through sexual hybridization and self-compatibility test,pollen viability and sterility was studied;Microstructure changes of pollen and stigma were also observed in blooming stage.The results showed that:(1)Floret epidermis structure of Den.hercoglossum was different and complex,epidermis of labellum,pollen cap and sepal were covered with protuberance and barb,but petal epidermis was smooth;(2)Hybridization of ... |
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| Keywords: | Dendrobium hercoglossum stigma pollination hybridization sterility |
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