| 聚乙二醇对东北红豆杉培养细胞的 |
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| 引用本文： | 陈正山,王勤.聚乙二醇对东北红豆杉培养细胞的[J].西北植物学报,2001,21(2):257-261. |
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| 作者姓名： | 陈正山 王勤 |
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| 作者单位： | 兰州大学生命科学学院生物化学和分子生物学系，兰州 730000 |
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| 摘 要： | 在改良的B
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| 关 键 词： | 东北红豆杉 紫杉醇 聚乙二醇 高压液相色谱仪 |
| 文章编号： | 1000-4025-(2001)02-0257-05 |
| 修稿时间： | 1999年11月1日 |
Effects of polyethylene
glycol on cell growth and taxol Effects of polyethylene glycol on cell growth and taxol |
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| Abstract: | Cells of Taxus cuspidata were suspensively cultured in modifiedB5 medium supplemented with different concentratioin PEG (Polyethylene glycol).Great effects of PEG on cell growth and taxol production were observed by determining fresh weight and dry weight of cells and taxol contents was determined with HPLC at different days of culture.10 g/L PEG is the best concentration for cell growth.The highest biomass was achieved in 16 days culture with 28.73 g/flask of fresh weight and 2.14 g/flask of dry weight,which increasing 3.8 times and 3.1 times respectively.20 g/L PEG is good for accumulation of taxol.Taxol concentration in medium amounted to the highest levels with 2 350 μg/L when the cells were cultured for 25 days,which is 11 times of that of PEG-free |
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