卡那霉素对地灵愈伤组织诱导和生长的影响

卡那霉素对地灵愈伤组织的诱导和生长均有抑制作用，随着卡那霉素浓度增加，愈伤组织形成的比率降低，质量增加幅度减小。不同的外植体对卡那霉素的敏感度不同，茎的敏感度明显高于叶片。当浓度超过30mg/L时，茎愈伤组织的诱导及生长几乎完全受到抑制。因此，在地灵的遗传转化中，30mg/L是卡那霉素的适宜浓度。

Effect of Kanamycin on induction and growth of Stachys sieboldii callus

Kanamycin inhibits the formation and the proliferation of Stachys sieboldii callus. With the increase of Kanamycin concentrations,the frequency of callus formation and the increase of weight are lower. The sensitivity of leaves and stems is different and obviously, the stem's is higher than the leave's. When the Kanamycin concentration is over 30 mg/L,the formation and the proliferation of the stems callus are inhibited. So we think the optimum Kanamycin concentration is about 30 mg/L for S. sieboldii genetic transformation.