| TDZ诱导甘露子茎段高频再生 |
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| 引用本文： | 李卫,董文,等.TDZ诱导甘露子茎段高频再生[J].西北植物学报,2002,22(4):965-969. |
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| 作者姓名： | 李卫 董文 |
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| 作者单位： | 兰州大学细胞生物学研究所,兰州,730000 |
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| 摘 要： | 以茎段为外植体，经TDZ诱导后，建立了甘露子的高频再生体系，其中以附加1．0mg/L TDZ的效果最好，经过8周的培养平均每个外植体可以获得37．5个不定芽，在MS培养基上生根以后，经过炼苗的再生植株可有效地定植于土壤中，组织学观察表明再生植株多数是通过器官发生途径获得的，也有少数是通过体胚途径发生的。
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| 关 键 词： | TDZ诱导 甘露子 茎段 高频再生 草药 |
| 文章编号： | 1000-4025(2002)04-0965-05 |
| 修稿时间： | 2001年9月5日 |
TDZ induced high frequency of plantlet regeneration from axillary node of Stachys sieboldii |
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| LI Wei,DONG Wen,ZHAO Dong li,GUO Guang qin,ZHENG Guo chang.TDZ induced high frequency of plantlet regeneration from axillary node of Stachys sieboldii[J].Acta Botanica Boreali-Occidentalia Sinica,2002,22(4):965-969. |
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| Authors: | LI Wei DONG Wen ZHAO Dong li GUO Guang qin ZHENG Guo chang |
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| Abstract: | Using axillary nodes as explants,a high frequency plantlet regeneration system induced by thidiazuron(TDZ) was established.After 8 weeks of culture on MS medium containing 1.0 mg/L TDZ,37.5 adventitious shoots per explant on average were achieved.Rooted plantlets were successfully transplanted to soil after acclimatization.Histological examination showed that most of the plantlets were regenerated via organogenesis,but some of them via somatic embryogenesis. |
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| Keywords: | Stachys sieboldii (Miq ) TDZ regeneration |
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