利用微嫁接技术促进麻疯树再生不定芽的生长

该研究以麻疯树种子实生苗的小芽和芽条为接穗,以带有胚根的实生苗下胚轴为砧木进行无菌微嫁接,试图建立新的有效微嫁接方法,解决农杆菌介导的麻疯树遗传转化体系中再生的转化不定芽难以顺利发育成完整植株的问题。结果表明:(1)抗生素对嫁接苗的生长有显著的抑制作用。(2)进行微嫁接所用砧木的苗龄以5d为宜。(3)进行微嫁接时适宜采用的砧木类型为带有部分胚根的下胚轴。(4)嫁接苗在0.3mg/L IBA+2mg/L谷氨酰胺+1/2MS培养基上的生长效果最好。(5)嫁接苗的移栽成活率最高可达76.40%。(6)以小芽或芽条为接穗的嫁接苗均可正常生长。该研究建立的麻疯树微嫁接体系,为解决麻疯树转化不定芽或芽条生长发育困难的问题提供了一条新的途径。

Development of an in vitro Micrografting Method for the Enhancement of Growth of Isolated Shoots and Buds in Jatropha curcas L.

Adventitious buds regenerated through Agrobacterium-mediated Jatropha curcas L.transformation systems are usually difficult to develop to whole plantlets.In order to solve the problem,we tested in vitro micrografting methods by using J.curcas buds and shoots isolated from seedlings as scions and the lower part of J.curcas seedlings as stocks.The experimental results showed that:(1)Antibiotics could hinder the further growth of grafted seedlings significantly;(2)The most suitable stocks were those prepared from 5-day-old seedlings;(3)The hypocotyls with the upper part of the radicals were the most appropriate type of stocks;(4)Significant enhancement on the growth of buds in culture was confirmed in 1/2 MS medium containing 0.3 mg/L indole-3-butyric acid (IBA) and 2 mg/L L-glutamine (Gln);(5)The grafted seedlings could be transplanted to soil with quite high survival rate (76.40%);(6) The grafted seedlings with shoots or buds served as scions could grow normally.The growth of the transformed buds and shoots could be enhanced effectively by making use of the established in vitro micrografting system.